1887

Abstract

Transfer of RP4::Mu plasmids from K12 to EBF65/65 was very inefficient compared with RP4 and was only detectable to strains of EBF65/65 lacking pAV2, a cryptic plasmid thought to code for a restriction/modification system. RP4::Mu-derived plasmids transferred from pAV2strains of EBF65/65 back to K12 were found to carry defective prophages which had lost the abilityto produce detectable phage particles. Re-transfer of these defective plasmids from and strains of K12 back to EBF65/65, when compared with the transfer of RP4, provided evidence for a second restriction/modification system in EBF65/65 which affected mainly Mu DNA. Using a mutant Mu prophage, Mu , it was possible to obtain RP4::Mu plasmids in EBF65/65 which were non-defective. These produced viable Mu particles when transferred back to K12 and could also be thermoinduced in EBF65/65; however, expression of Mu in EBF65/65 was very poor and plaques were only detected on a restrictionless strain of K12. ‘Plasmid suicide by Mu’ may enable the development of a method for directed chromosome mobilization in

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1980-12-01
2021-05-12
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References

  1. Barth P. T., Grinter N. J. 1974; Comparison of the deoxyribonucleic acid molecular weights and homologies of plasmids conferring linked resistance to streptomycin and sulphonamides. Journal of Bacteriology 120:618–630
    [Google Scholar]
  2. Boucher C., Bergeron B., Barate De Bertalmio M., DÉnariÉ J. 1977; Introduction of bacteriophage Mu into Pseudomonas solanacearum and Rhizobium meliloti using the R factor RP4. Journal of General Microbiology 98:253–263
    [Google Scholar]
  3. Bukhari A. I., Ljungquist E. 1977; Bacteriophage Mu: methods for cultivation and use. In DNA Insertion Elements, Plasmids, and Episomes pp. 749–756 Bukhari A. I., Shapiro J. A., Adhya S. L. Edited by New York:: Cold Spring Harbor Laboratory;
    [Google Scholar]
  4. Coplin D. L. 1979; Introduction of bacteriophage Mu into Erwinia stewartii by use of an RK2::Mu hybrid plasmid. Journal of General Microbiology 113:181–184
    [Google Scholar]
  5. Datta N., Hedges R. W., Shaw E. J., Sykes R. B., Richmond M. H. 1971; Properties of an R factor from Pseudomonas aeruginosa. Journal of Bacteriology 108:1244–1249
    [Google Scholar]
  6. De Graaf J., Kreuning P. C., Van De Putte P. 1973; Host-controlled restriction and modification of bacteriophage Mu and Mu-promoted chromosome mobilization in Citrobacter freundii. Molecular and General Genetics 123:283–288
    [Google Scholar]
  7. DÉnariÉ J., Rosenberg C., Bergeron B., Boucher C., Michel M., Barate De Bartalmio M. 1977; Potential of RP4::Mu plasmids for in vivo genetic engineering of Gram-negative bacteria. In DNA Insertion Elements, Plasmids, and Episomes pp. 507–520 Bukhari A. I., Shapiro J. A., Adhya S. L. Edited by New York:: Cold Spring Harbor Laboratory;
    [Google Scholar]
  8. Hinchliffe E., Nugent M. E., Vivian A. 1980; Naturally occurring plasmids in Acinetobacter calcoaceticus: pAV2, a plasmid which influences the fertility of the sex factor pAV1. Journal of General Microbiology 121:411–418
    [Google Scholar]
  9. Howe M., Bade E. 1975; The genetics and molecular biology of bacteriophage Mu-1. Science 190:624–632
    [Google Scholar]
  10. Towner K. J. 1978; Chromosome mapping in Acinetobacter calcoaceticus. Journal of General Microbiology 104:175–180
    [Google Scholar]
  11. Towner K. J., Vivian A. 1976a; RP4-mediated conjugation in Acinetobacter calcoaceticus. Journal of General Microbiology 93:355–360
    [Google Scholar]
  12. Towner K. J., Vivian A. 1976b; RP4 fertility variants in Acinetobacter calcoaceticus. Genetical Research 28:301–306
    [Google Scholar]
  13. Towner K. J., Vivian A. 1977; Plasmids capable of transfer and chromosome mobilization in Acinetobacter calcoaceticus. Journal of General Microbiology 101:167–171
    [Google Scholar]
  14. Van De Putte P., Gruijthuijsen M. 1972; Chromosome mobilization and integration of F factors in the chromosome of recA strains of E. coli under the influence of bacteriophage Mu-1. Molecular and General Genetics 118:173–183
    [Google Scholar]
  15. Van Vliet F., Silva B., Van Montagu M., Schell J. 1978; Transfer of RP4:: Mu plasmids to Agrobacterium tumefaciens. Plasmid 1:446–455
    [Google Scholar]
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