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The naturally occurring plasmid pAV2 restricts the entry of the P class plasmid RP4 and the W class plasmids R388 and S-a into Acinetobacter calcoaceticus strain EBF65/65 from Escherichia coli. The W class plasmids only transfer from E. coli into pAV2− strains. Plasmid RP4 is modified in the presence of pAV2 such that it is no longer restricted on entry into pAV2 recipients of strain EBF65/65.
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