S288C/1 was grown in a glucose-limited chemostat at population doubling times (τ) of 80 to 736 min. Estimates of the daughter cycle time (D), the parent cycle time (P) and the budded period (B) were obtained from bud scar analyses and equations derived from the Hartwell & Unger model of asymmetric cell division. D, P and B all showed biphasic linear relationships to τ, quantitatively different from estimates for the same strain in batch culture. Median cell volume and dry weight per cell increased at the faster growth rates, but the average cell density reached a minimum at τ = 150 min. The contiguous array of bud scars on parent cells became increasingly irregular as the doubling time increased from 140 min. At the fastest growth rates a small percentage of filamentous forms were observed due to failure of cells to undergo cell separation.


Article metrics loading...

Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error