The isolation and pathogenic response of various drug-resistant and auxotrophic mutants of strain PgB3 of , the causal agent of bacterial blight of soybean, are reported. A mutagenesis procedure is described which allows efficient recovery of spontaneous, ethyl methanesulphonate-, hycanthone-, 2-aminopurine- and -methyl--nitro--nitrosoguanidine-induced mutants. While many of the auxotrophic mutants were avirulent, fully virulent histidine, adenine and methionine auxotrophic mutants were isolated. The virulence of certain methionine-requiring mutants did not correlate with the amount of methionine required by the mutant. In every instance where revertants to prototrophy were isolated, the revertant regained wild-type virulence.


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