1887

Abstract

Cells of RodB changed from rods to cocci when shifted from 20 to 42 °C in media containing no additional anions. Quantitative studies of surface growth, including cross-wall formation and pole construction, have been made from reconstructions obtained from central, longitudinal sections of cells. Measurements of surface area and volume were obtained by mathematical rotation of axial sections about their longitudinal axis. Surface markers, perhaps analogous to the wall bands of streptococci, have been used to distinguish septal from cylindrical wall. During the shape change, wall volume increased most rapidly, in relation to cell volume, at the division site. The average volume of wall distal to the septum also increased but the slopes of the lines relating distal wall volume to cell volume were the same at all stages of the shape change. The quantity of wall per distal pole gradually declined with increase of cell volume and as the cells became more coccus-like. Collectively, these features suggest that wall continues to be produced at sites of cylindrical extension but fails to become incorporated into the existing cylinder to maintain a constant diameter. Instead, wall material may be used to thicken the surface of distal poles, but the rate of addition may decline as the cells assume a coccal morphology. The change from rods to cocci may involve a progressive dependence on septal growth for surface expansion and a modification of the time at which the cross-wall is closed. Septal closure is progressively delayed as the organisms change into cocci, so that cross-wall separation precedes septal closure, as in streptococci.

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/content/journal/micro/10.1099/00221287-121-1-93
1980-11-01
2024-11-03
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References

  1. Armitage P. 1977 Statistical Methods in Medical Research Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  2. Boylan R.J., Mendelson N.H. 1969; Initial characterisation of a temperature-sensitive Rodmutant of Bacillus subtilis. Journal of Bacteriology 100:1316–1321
    [Google Scholar]
  3. Burdett I.D.J. 1979; Electron microscope study of the rod-to-coccus shape change in a temperature-sensitive Rod mutant of Bacillus subtilis. Journal of Bacteriology 137:1395–1405
    [Google Scholar]
  4. Burdett I.D.J., Higgins M.L. 1978; Study of pole assembly in Bacillus subtilis by computer reconstruction of septal zones seen in central, longitudinal thin sections of cells.. Journal of Bacteriology 133:959–971
    [Google Scholar]
  5. Cole R.M., Popkin T.J., Boylan R.J., Mendelson N.H. 1970; Ultrastructure of a temperature-sensitive Rod mutant of Bacillus subtilis. Journal of Bacteriology 103:793–810
    [Google Scholar]
  6. Green R.J., Perkins W.J., Piper E.A., Stenning B.F. 1979; The transfer of selected image data to a computer using a conductive tablet.. Journal of Biomedical Engineering 1:240–246
    [Google Scholar]
  7. Higgins M.L. 1976; Three-dimensional reconstruction of whole cells of Streptococcus faecalis from thin sections of cells.. Journal of Bacteriology 127:1337–1345
    [Google Scholar]
  8. Higgins M.L., Shockman G.D. 1970; Model for cell wall growth of Streptococcus faecalis. Journal of Bacteriology 101:643–648
    [Google Scholar]
  9. Higgins M.L., Shockman G.D. 1976; Study of a cycle of cell wall assembly in Streptococcus faecalis by three-dimensional reconstructions of thin sections of cells.. Journal of Bacteriology 127:1346–1358
    [Google Scholar]
  10. Reeve J.N., Mendelson N.H., Cole R.M. 1972; Cell morphology of Bacillus subtilis: the effect of genetic background on the expression of a Rod gene.. Molecular and General Genetics 119:11–26
    [Google Scholar]
  11. Rogers H.J., Thurman P.F. 1978; Temperature-sensitive nature of the rodB mutation in Bacillus subtilis. Journal of Bacteriology 133:298–305
    [Google Scholar]
  12. Rogers H.J., Mcconnell M.M., Burdett I.D.J. 1968; Cell wall or membrane mutants of Bacillus subtilis and B. licheniformis with grossly disturbed morphology.. Nature, London 219:285–288
    [Google Scholar]
  13. Rogers H.J., Mcconnell M.M., Burdett I.D.J. 1970; The isolation and characterization of mutants of Bacillus subtilis and B. licheniformis with disturbed morphology and cell division.. Journal of General Microbiology 61:155–171
    [Google Scholar]
  14. Rogers H.J., Thurman P.F., Buxton R.S. 1976; Magnesium and anion requirements of rodB mutants of Bacillus subtilis. Journal of Bacteriology 125:556–564
    [Google Scholar]
  15. Rogers H.J., Ward J.B., Burdett I.D.J. 1978; Structure and growth of the walls of Gram-positive bacteria.. Symposia of the Society for General Microbiology 28:139–176
    [Google Scholar]
  16. Shiflett M.A., Brooks D., Young F.E. 1977; Cell wall and morphological changes induced by temperature-shift in Bacillus subtilis cell wall mutants.. Journal of Bacteriology 132:681–690
    [Google Scholar]
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