1887

Abstract

Rapid development of competence can be induced in cultures of by incubation at 37 °C after previous growth at 42 °C. This temperature-induced competence was accompanied by an increase in DNA binding capacity and breakdown of donor DNA. Inhibition of protein synthesis prevented the rapid increase of competence. This is probably due to the inhibition of synthesis of a constituent that enables the bacteria to bind DNA.

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1980-11-01
2024-04-23
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References

  1. Akrigg A., Ayad S.R., Blamire J. 1969; Uptake of DNA by competent bacteria - a possible mechanism. Journal of Theoretical Biology 24:266–272
    [Google Scholar]
  2. Davidoff-Abelson R., Dubnau D. 1973; Kinetic analysis of the products of donor deoxyribonucleate in transformed cells of B. subtilis. Journal of Bacteriology 116:154–162
    [Google Scholar]
  3. Dooley D.C., Hadden C.T., Nester E.W. 1971; Macromolecular synthesis in Bacillus subtilis during development of the competent state. Journal of Bacteriology 108:668–679
    [Google Scholar]
  4. García E., Lopez P., Perezureña M.T., Espinosa M. 1978; Early stages in Bacillus subtilis transformation: association between homologous DNA and surface structures. Journal of Bacteriology 135:731–740
    [Google Scholar]
  5. Joenje H., Venema G. 1975; Different nuclease activities in competent and noncompetent Bacillus subtilis. Journal of Bacteriology 122:25–33
    [Google Scholar]
  6. Joenje H., Admiraal W., Venema G. 1973; Isolation and partial characterization of a temperature-sensitive mutant of Bacillus subtilis impaired in the development of competence for genetic transformation. Journal of General Microbiology 78:67–77
    [Google Scholar]
  7. Joenje H., Konings W.N., Venema G. 1974; Interactions between exogenous deoxyribonucleic acid and membrane vesicles isolated from Bacillus subtilis 168. Journal of Bacteriology 119:784–794
    [Google Scholar]
  8. Lacks S. 1977; Binding and entry of DNA in pneumococcal transformation. In Modern Trends in Bacterial Transformation and Transfection pp. 35–44 Edited by Porters A., Lopez R., Espinosa M. Amsterdam: North Holland Publishing Co;
    [Google Scholar]
  9. Lacks S., Neuberger M. 1975; Membrane location of a deoxyribonuclease implicated in the genetic transformation of Diplococcus pneumoniae. Journal of Bacteriology 124:1321–1329
    [Google Scholar]
  10. Lacks S., Greenberg B., Neuberger M. 1974; Role of a deoxyribonuclease in the genetic transformation of Diplococcus pneumoniae. Proceedings of the National Academy of Sciences of the United States of America 71:2305–2309
    [Google Scholar]
  11. Spizizen J. 1958; Transformation of biochemically deficient strains of Bacillus subtilis by deoxyribo- nucleate. Proceedings of the National Academy of Sciences of the United States of America 44:1072–1078
    [Google Scholar]
  12. Seto H., Tomasz A. 1974; Early stages in DNA binding and uptake during genetic transformation of pneumococci. Proceedings of the National Academy of Sciences of the United States of America 71:1493–1498
    [Google Scholar]
  13. Tomasz A. 1973; Interactions between cells and DNA molecules: the nature of the competent state. In Bacterial Membranes and Walls pp. 322–352 Edited by Leive L. New York: Marcel Dekker;
    [Google Scholar]
  14. Wilson G.A., Bott K.F. 1968; Nutritional factors influencing the development of competence in the Bacillus subtilis transformation system. Journal of Bacteriology 95:1439–1449
    [Google Scholar]
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