1887

Abstract

From a strain carrying , a mutation giving rise to asporogeny, a revertant was isolated which sporulated at about 10 of the wild-type frequency but in which the time-course of sporulation was much protracted. Genetic analysis of this revertant showed that it retained but had acquired a secondary mutation failed to suppress mutations in or ; it also failed to suppress another mutation in the locus. is extremely closely linked (recombination frequency ⩽ 1%) with the mutation that it specifically suppresses. Strains carrying alone sporulated at a frequency at least two orders of magnitude below that in the double mutant. It is suggested that and lead to compensating amino acid changes in the protein specified by the locus.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-121-1-69
1980-11-01
2021-08-04
Loading full text...

Full text loading...

/deliver/fulltext/micro/121/1/mic-121-1-69.html?itemId=/content/journal/micro/10.1099/00221287-121-1-69&mimeType=html&fmt=ahah

References

  1. Ayad S.R., Barker G.R. 1969; The integration of donor and recipient deoxyribonucleic acid during transformation of Bacillus subtilis. Biochemical Journal 113:167–174
    [Google Scholar]
  2. Coote J.G. 1972; Sporulation in Bacillus subtilis. Genetic analysis of oligosporogenous mutants. Journal of General Microbiology 71:17–27
    [Google Scholar]
  3. Coote J.G., Mandelstam J. 1973; Use of constructed double mutants for determining the temporal order of expression of sporulation genes in Bacillus subtilis. Journal of Bacteriology 114:1254–1263
    [Google Scholar]
  4. Ephrati-Elizur E. 1968; Spontaneous transformation in Bacillus subtilis. Genetical Research 11:83–96
    [Google Scholar]
  5. Grodzicker T., Zipser D. 1968; A mutation which creates a new site for the re-initiation of polypeptide synthesis in the Z gene of the lac operon of Escherichia coli. Journal of Molecular Biology 38:305–314
    [Google Scholar]
  6. Helinski D.R., Yanofsky C. 1963; A genetic and biochemical analysis of second site reversion. Journal of Biological Chemistry 238:1043–1048
    [Google Scholar]
  7. Mandelstam J. 1976; Bacterial sporulation: a problem in the biochemistry and genetics of a primitive developmental system. Proceedings of the Royal Society of London B 193:89–106
    [Google Scholar]
  8. Marmur J. 1961; A procedure for the isolation of deoxyribonucleic acid from micro-organisms. Journal of Molecular Biology 3:208–218
    [Google Scholar]
  9. Piggot P.J. 1973; Mapping of asporogenous mutations of Bacillus subtilis: a minimum estimate of the number of sporulation operons. Journal of Bacteriology 114:1241–1253
    [Google Scholar]
  10. Piggot P.J., Coote J.G. 1976; Genetic aspects of bacterial endospore formation. Bacteriological Reviews 40:908–962
    [Google Scholar]
  11. Piggot P.J., Taylor S.Y. 1977; New types of mutation affecting formation of alkaline phosphatase by Bacillus subtilis in sporulation conditions. Journal of General Microbiology 102:69–80
    [Google Scholar]
  12. Sterlini J.M., Mandelstam J. 1969; Commitment to sporulation in Bacillus subtilis and its relationship to development of actinomycin resistance. Biochemical Journal 113:29–37
    [Google Scholar]
  13. Takahashi I. 1963; Transducing phages for Bacillus subtilis. Journal of General Microbiology 31:211–217
    [Google Scholar]
  14. Trowsdale J., Chen S.M.H., Hoch J.A. 1979; Genetic analysis of a class of polymixin resistant partial revertants of stage O sporulation mutants of Bacillus subtilis: map of the chromosome region near the origin of replication. Molecular and General Genetics 173:61–70
    [Google Scholar]
  15. Waites W.M., Kay D., Dawes I.W., Wood D.A., Warren S.C., Mandelstam J. 1970; Sporulation in Bacillus subtilis. Correlation of biochemical events with morphological changes in asporogenous mutants. Biochemical Journal 118:667–676
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-121-1-69
Loading
/content/journal/micro/10.1099/00221287-121-1-69
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error