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A transposon, Tn733, encoding the gentamicin acetyltransferase AAC(3) was found on two gentamicin R plasmids of IncW at Hammersmith Hospital. Transposon Tn733 has a molecular mass of 5·8 megadaltons and gives a characteristic 2·4 megadalton fragment on digestion with Eco RI. The appearance of gentamicin resistance on a transposon will increase the chances of spread of this gene.
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