1887

Abstract

Using microdensitometry, the cell cycle was investigated in large populations (500) of Feulgen-stained nuclei in conidia, hyphae and resting structures of various strains (all haploid) of and as well as a stable diploid strain, var. When uninucleate conidia, harvested from 3 d Roux bottle agar cultures, were incubated in stationary liquid complete medium at 24 C in the dark, they exhibited partial synchrony for germ-tube emergence (50% emergent at about 7 h, reaching 95% at 10 to 12 h), DNA replication (50% increase at about 7.5 h) and mitosis (50% at about 10 h, reaching 95% at 12 h); the second nuclear division occurred at 21 to 24 h. Significant amounts of DNA replication were not detected until 6 h; maximum levels, i.e. a doubling of DNA content (in nuclei not yet divided), occurred at 10 h. There was a 3 h G1 phase between the first and second nuclear divisions; the S and G2 phases were estimated at about 30 and 90 min duration, respectively. The absolute DNA content per nucleus for ungerminated conidia of one strain of was found to be 0.025 to 0.030 pg. In growing hyphae, only the apical cell(s) contained two or more nuclei; DNA levels approximated to those for ungerminated conidia (G1) but a small number of nuclei had begun the S phase. In hyphal tip regions comprising up to 10 cells, a synchronous nuclear division was observed, and nuclei in the apical cells often had higher DNA values than other nuclei in the same hypha. Cells of young microsclerotia (6 to 8 d-old) of and resting mycelium (9 to 12 d-old) of were haploid and uninucleate with a small minority of the nuclei in the S phase; no evidence for diploidy or endopolyploidy was found at these sampling levels.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-121-1-231
1980-11-01
2021-07-29
Loading full text...

Full text loading...

/deliver/fulltext/micro/121/1/mic-121-1-231.html?itemId=/content/journal/micro/10.1099/00221287-121-1-231&mimeType=html&fmt=ahah

References

  1. Alfert M., Das N.K. 1969; Evidence for control of the rate of nuclear DNA synthesis by the nuclear membrane in eukaryotic cells.. Proceedings of the National Academy of Sciences of the United States of America 63:123–128
    [Google Scholar]
  2. Bainbridge B.W. 1971; Macromolecular composition and nuclear division during spore germination in Aspergillus nidulans.. Journal of General Microbiology 66:319–325
    [Google Scholar]
  3. Bainbridge B.W. 1976; Estimation of the generation time and peripheral growth zone of Aspergillus nidulans and Alternaria solani hyphae from radial growth rates and ranges in apical cell length.. Journal of General Microbiology 97:125–127
    [Google Scholar]
  4. Clutterbuck A.J. 1969; Cell volume per nucleus in haploid and diploid strains of Aspergillus nidulans.. Journal of General Microbiology 55:291–299
    [Google Scholar]
  5. Clutterbuck A.J. 1970; Synchronous nuclear division and septation in Aspergillus nidulans.. Journal of General Microbiology 60:133–135
    [Google Scholar]
  6. Clutterbuck A.J., Roper J.A. 1966; A direct determination of nuclear distribution in heterokaryons of Aspergillus nidulans.. Genetical Research 7:185–194
    [Google Scholar]
  7. Cooke D.J., Dee J. 1974; Plasmodium formation without change in nuclear DNA content in Physarum polycephalum.. Genetical Research 23:307–318
    [Google Scholar]
  8. Gottlieb D., Van Etten J.L. 1964; Biochemical changes during the growth of fungi. I. Nitrogen compounds and carbohydrate changes in Penicillium atrovenetum.. Journal of Bacteriology 88:114–121
    [Google Scholar]
  9. Gottlieb D., Van Etten J.L. 1966; Changes in fungi with age. I. Chemical composition of Rhizoctonia solani and Sclerotium bataticola.. Journal of Bacteriology 91:161–168
    [Google Scholar]
  10. Haskins E.F., Therrien C.D. 1978; The nuclear cycle of the myxomycete Echinostelium minutum. I. Cytophotometric analysis of the nuclear DNA content of the amoebal and plasmodial phases.. Experimental Mycology 2:32–40
    [Google Scholar]
  11. Heale J.B. 1966; Heterokaryon synthesis and morphogenesis in Verticillium.. Journal of General Microbiology 45:419–427
    [Google Scholar]
  12. Heale J.B., Isaac I. 1965; Environmental factors in the production of dark resting structures in Verticillium albo-atrum, V. dahliae and V. tricorpus.. Transactions of the British Mycological Society 48:39–50
    [Google Scholar]
  13. Heale J.B., Gafoor A., Rajasingham K.C. 1968; Nuclear division in conidia and hyphae of Verticillium albo-atrum.. Canadian Journal of Genetics and Cytology 10:321–340
    [Google Scholar]
  14. Howard A., Pelc S.R. 1953; Synthesis of DNA in normal and irradiated cells and its relation to chromosome breakage.. Heredity 6: (Suppl.) 261
    [Google Scholar]
  15. Ingram R. 1968; Verticillium dahliae Kleb. var.longisporum Stark, a stable diploid.. Transactions of the British Mycological Society 51:339–441
    [Google Scholar]
  16. Kessel M., Rosenberger R.F. 1968; Regulation and timing of deoxyribonucleic acid synthesis in hyphae of Aspergillus nidulans.. Journal of Bacteriology 95:2275–2281
    [Google Scholar]
  17. Kihlman B.A., Hartley B. 1967; Subchromatid exchanges and the ‘folded fiber’ model of chromosome structure.. Hereditas 57:289–294
    [Google Scholar]
  18. King S.B., Alexander L.J. 1969; Nuclear behavior, septation and hyphal growth of Alternaria solani.. American Journal of Botany 56:249–253
    [Google Scholar]
  19. Kumari L., Decallone J.R., Meyer J.A. 1975; Deoxyribonucleic acid metabolism and nuclear division during spore germination in Fusarium oxysporum.. Journal of General Microbiology 88:245–252
    [Google Scholar]
  20. Lovett J. 1975; Growth and differentiation of Blastocladiella emersonii: cytodifferentiation and the role of ribonucleic acid and protein synthesis.. Bacteriological Reviews 39:345–404
    [Google Scholar]
  21. Macgarvie Q., Isaac I. 1966; Structure and behaviour of the nuclei of Verticillium spp.. Transactions of the British Mycological Society 49:687–693
    [Google Scholar]
  22. Maruyama Y., Alexander M. 1962; Distribution of protein and nucleic acids in hyphae and microconidia of Fusarium.. Archiv. für Microbiologie 41:401–407
    [Google Scholar]
  23. Rees H., Jinks J.L. 1952; The mechanism of variation in Penicillium heterokaryons.. Proceedings of the Royal Society B140:100–106
    [Google Scholar]
  24. Robinow C.F., Caten C.E. 1969; Mitosis in Aspergillus nidulans.. Journal of Cell Science 5:403–431
    [Google Scholar]
  25. Rosenberger R.F., Kessel M. 1967; Synchrony in nuclear replication in individual hyphae of Aspergillus nidulans.. Journal of Bacteriology 94:1464–1469
    [Google Scholar]
  26. Schmit J.C., Brody S. 1976; Biochemical genetics of Neurospora crassa conidial germination.. Bacteriological Reviews 40:1–41
    [Google Scholar]
  27. Therrien C.D., Yemma J.J. 1974; Comparative measurements of nuclear DNA in a heterothallic and a self-fertile isolate of the myxomycete, Didymium iridis.. American Journal of Botany 61:400–404
    [Google Scholar]
  28. Therrien C.D., Yemma J.J. 1975; Nuclear DNA content and ploidy values in clonally-developed plasmodia of the myxomycete Didymium iridis.. Caryologia 28:313–320
    [Google Scholar]
  29. Tolmsoff W.J. 1972; Diploidization and heritable gene repression-depression as a major source for variability in morphology, metabolism and pathogenicity of Verticillium species.. Phytopathology 62:407–413
    [Google Scholar]
  30. Tolmsoff W.J. 1973; Life cycles of Verticillium species.. In Verticillium Wilt of Cotton Proceedings of a Work Conference, National Cotton Pathology Research Laboratory, College Station, Texas 1971, (ARS-S-19) pp. 20–38
    [Google Scholar]
  31. Typas M.A., Heale J.B. 1976a; Acriflavine-induced hyaline variants in Verticillium alboatrum and V. dahliae.. Transactions of the British Mycological Society 66:15–25
    [Google Scholar]
  32. Typas M.A., Heale J.B. 1976b; Hetero-karyosis and the role of cytoplasmic inheritance of dark resting structure formation in Verticillium species.. Molecular and General Genetics 146:17–26
    [Google Scholar]
  33. Typas M.A., Heale J.B. 1977; Analysis of ploidy levels in strains of Verticillium using a Coulter counter.. Journal of General Microbiology 101:177–180
    [Google Scholar]
  34. Typas M.A., Heale J.B. 1978; Heterozygous diploid analyses via the parasexual cycle and a cytoplasmic pattern of inheritance in Verticillium species.. Genetical Research 31:131–144
    [Google Scholar]
  35. Typas M.A., Heale J.B. 1979; Transfer of a cytoplasmic factor by micro-injection in Verticillium.. Journal of General Microbiology 111:375–386
    [Google Scholar]
  36. Weijer J., Weisberg S.H. 1966; Karyokinesis of the somatic nucleus of Aspergillus nidulans.I. The juvenile chromosome cycle (Feulgen staining).. Canadian Journal of Genetics and Cytology 8:361–374
    [Google Scholar]
  37. Williamson D.H. 1965; The timing of DNA synthesis in the cell cycle of Saccharomyces cerevisiae.. Journal of Cell Biology 25:517–528
    [Google Scholar]
  38. Yemma J.J., Therrien C.D. 1972; Quantitative microspectrophotometry of nuclear DNA in selfing strains of the myxomycete Didymium iridis.. American Journal of Botany 59:828–835
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-121-1-231
Loading
/content/journal/micro/10.1099/00221287-121-1-231
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error