The lethal and mutagenic effects of ultraviolet light (u.v.), ethyl methanesulphonate (EMS), methyl methanesulphonate (MMS), N-methyl-N′-nitro-N-nitrosoguanidine (MNNG) and mitomycin C on the unicellular cyanobacterium Gloeocapsa alpicola have been investigated. Mitomycin C and EMS did not significantly increase the frequency of streptomycin-resistant mutants above the spontaneous level. MNNG and MMS produced increases of 10 to 30 times the spontaneous frequency. Contrary to previous reports on mutation induction in cyanobacteria, u.v. was the most effective mutagen, increasing the spontaneous mutation frequency 4000-fold. However, since photoreactivating light eliminated most of this mutagenic response it was necessary to rigorously exclude light of wavelengths < 550 nm during post-irradiation growth. Caffeine failed to increase the mutation frequency of u.v.-irradiated cultures, but cells were more sensitive to u.v. killing in the presence of caffeine.
BridgesB.A.1976; Mutation induction.. In Second International Symposium on the Genetics of Industrial Microorganisms pp. 7–14MacdonaldK.D. Edited by London:: Academic Press.;
DelaneyS.F., HerdmanM., CarrN.G.1976; Genetics of blue-green algae.. In Genetics of Algae pp. 7–29LewinR.A. Edited by Oxford:: Blackwell Scientific Publications.;
HerdmanM., DelaneyS.F., CarrN.G.1973; A new medium for the isolation and growth of auxotrophic mutants of the blue-green alga Anacystis nidulans.. Journal of General Microbiology 79:233–237
IngramL.O., PiersonD., KaneJ.F., Van BaalenC.1972; Documentation of auxotrophic mutation in blue-green bacteria: characterization of a tryptophan auxotroph in Agmenellum quadruplicatum.. Journal of Bacteriology 111:112–118
IshiiY., KondoS.1975; Comparative analysis of deletion and base-change mutabilities of Escherichia coli B strains differing in DNA , repair capacity (wild-type, uvrA−, polA−, recA−) by various mutagens.. Mutation Research 27:27–44
KaneyA.R.1973; Enzymatic characterization of a phenylalanine auxotroph of the blue-green bacterium Synechococcus cedrorum.. Archiv für Mikrobiologie 92:139–142
ShestakovS.V.1972; Mutagenesis and repair systems in unicellular blue-green algae.. In Taxonomy and Biology of Blue-Green Algae pp. 262–263DesikacharyT.V. Edited by Madras:: University of Madras.;
ShestakovS.V., JevnerV.D.1968; Study of mutagenesis in the blue-green alga Anacystis nidulans.. Proceedings of the XII International Congress of Genetics 1:84
SinghR.N., TiwariD.N.1969; Induction by ultraviolet irradiation of mutation in the bluegreen alga Nostoc linckia (Roth) Born, et Flah.. Nature; London: 22162–64
SrivastavaB.S.1969; Ultraviolet induced mutations to growth factor requirement and penicillin resistance in a blue-green alga.. Archiv für Mikrobiologie 66:234–238
WilliamsE., LambertJ., O’BrienP.A., HoughtonJ.A.1979; Evidence for dark repair of ultraviolet light damage in the blue-green alga Gloeocapsa alpicola.. Photochemistry and Photobiology 29:543–547
ZhevnerV.D., ShestakovS.V.1972; Studies on ultraviolet sensitive mutants of the blue-green alga Synechocystis aquatilis Sanv.. Archiv für Mikrobiologie 86:349–360