1887

Abstract

A continuous-flow culture selection experiment was designed to obtain mutants of strain PP3 capable of growing on 2-monochlorobutanoic acid (2MCBA), normally unable to act as a carbon and energy source for PP3. The experiment involved continuous exposure of PP3 to 2MCBA whilst the population's growth was supported by the metabolizable substrate, 2-monochloropropionic acid. The system failed to select for mutants able to grow on 2MCBA alone. However, the procedure resulted in the selection of two different strains of , namely, PP309 and PP310, both stably maintained as a mixed culture having excluded the parent strain. The two mutants differed significantly from PP3 with respect to their dehalogenating system since it was found that major changes in both dehalogenase specific activities and overall activity ratios had occurred. The dehalogenase system in strains PP309 and PP310 was inducible as in the parent strain. The results obtained were explained in terms of elevated production of fraction I dehalogenase compared with fraction II dehalogenase in the mutant strains in contrast to the parent strain. The results demonstrate that mutants may be selected showing altered enzyme activities in the absence of acquiring the capacity to grow solely on a novel substrate which provided the selection pressure.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-121-1-187
1980-11-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/micro/121/1/mic-121-1-187.html?itemId=/content/journal/micro/10.1099/00221287-121-1-187&mimeType=html&fmt=ahah

References

  1. Bull A.T., Senior E., Slater J.H. 1976; Concerning the evolution of metabolic capabilities in bacteria: Dalapon metabolism by Pseudomonas putida. . Proceedings of Society for General Microbiology 4:39
    [Google Scholar]
  2. Clarke P.H., Lilly M.D. 1969; The regulation of enzyme synthesis during growth.. Symposia of the Society for General Microbiology 19:113–159
    [Google Scholar]
  3. Dean A.C.R. 1972; Influence of environment on the control of enzyme synthesis.. Journal of Applied Chemistry and Biotechnology 22:245–259
    [Google Scholar]
  4. Fairbanks G., Steck T.L., Wallach D.F.H. 1971; Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane.. Biochemistry 10:2606–2617
    [Google Scholar]
  5. Gornall A.G., Bardawill C.J., David M.M. 1948; Determination of serum proteins by means of the biuret reaction.. Journal of Biological Chemistry 177:751–766
    [Google Scholar]
  6. Hartley B.S. 1974; Enzyme families.. Symposia of the Society for General Microbiology 24:151–182
    [Google Scholar]
  7. Hartley B.S., Burleigh B.D., Midwinter G.G., Moore C.H., Morris H.R., Rigby P.W.J., Smith M.J., Taylor S.S. 1972; Where do new enzymes come from?. In Enzymes: Structure and Function pp. 151–176 Drenth J., Oosterbaan R.A., Veeger C. Edited by Amsterdam:: North-Holland.;
    [Google Scholar]
  8. Horiuchi T., Tomizawa J., Novick A. 1962; Isolation and properties of bacteria capable of high rates of β-galactosidase synthesis.. Biochimica et biophysica acta 55:152–163
    [Google Scholar]
  9. King A., Phillips I. 1978; The identification of pseudomonads and related bacteria in a clinical laboratory.. Journal of Medical Microbiology 11:165–176
    [Google Scholar]
  10. Laemmli U.K. 1970; Cleavage of structural proteins during the asse. Nature; London: 227680–685
    [Google Scholar]
  11. Senior E., Bull A.T., Slater J.H. 1976; Enzyme evolution in a microbial community growing on the herbicide Dalapon.. Nature London: 262:476–479
    [Google Scholar]
  12. Slater J.H., Lovatt D., Weightman A.J., Senior E., Bull A.T. 1979; The growth of Pseudomonas putida on chlorinated aliphatic acids and its dehalogenase activity.. Journal of General Microbiology 114:125–136
    [Google Scholar]
  13. Weightman A.J., Slater J.H., Bull A.T. 1979a; Cleavage of the carbon-chlorine bond by Pseudomonas putida. . Society for General Microbiology Quarterly 6:76–77
    [Google Scholar]
  14. Weightman A.J., Slater J.H., Bull A.T. 1979b; The partial purification of two dehalogenases from Pseudomonas putida PP3.. FEMS Microbiology Letters 6:231–234
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-121-1-187
Loading
/content/journal/micro/10.1099/00221287-121-1-187
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error