1887

Abstract

The intracellular ATP concentration in plasmodia of the myxomycete was determined by the luciferin-luciferase method after stimulation with various chemotactic chemicals. Repellent salts induced an ATP increase. The change in ATP concentration paralleled changes in contraction and in electrical potential with respect to both time course and concentration dependence. Other repellents (certain organic chemicals and high osmolarity) did not induce appreciable change in ATP concentration within 15 min, but prolonged treatment with hydrophobic repellents and DO led to diminished ATP. None of the attractants examined (e.g. glucose, alanine, KHPO and 2-deoxyglucose) changed the ATP concentration. A possible role of ATP as a mediator in response to salts is discussed.

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/content/journal/micro/10.1099/00221287-121-1-175
1980-11-01
2021-07-29
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References

  1. Ataka M., Tsuchii A., Ueda T., Kurihara K., Kobatake Y. 1978; Comparative studies on the reception of bitter stimuli in the frog, Tetra- hymena, slime mold and Nitella.. Comparative Biochemistry and Physiology 61A:109–115
    [Google Scholar]
  2. Beutler E., Baluda M. 1964; Simplified determination of blood adenosine triphosphate using the firefly system.. Blood 23:688–698
    [Google Scholar]
  3. Camp W.G. 1936; A method of cultivating myxomycete plasmodia.. Bulletin of the Torrey Botanical Club 63:205–210
    [Google Scholar]
  4. Carlile M.J. 1970; Nutrition and chemotaxis in the myxomycete Physarum polycephalum: the effect of carbohydrates on the plasmodium.. Journal of General Microbiology 63:221–226
    [Google Scholar]
  5. Chet I., Naveh A., Henis Y. 1977; Chemotaxis of Physarum polycephalum towards carbohydrates, amino acids and nucleotides.. Journal of General Microbiology 102:145–148
    [Google Scholar]
  6. Curtin N.A., Davies R.E. 1973; Chemical and mechanical changes during stretching of activated frog skeletal muscle.. Cold Spring Harbor Symposia on Quantitative Biology 37:619–626
    [Google Scholar]
  7. Götz Von Olenhusen K., Wohlfarth-Botter-mann K.E. 1979; Effects of caffeine and D20 on persistence and de novo generation of intrinsic oscillatory contraction automaticity in Physarum.. Cell and Tissue Research 197:479–499
    [Google Scholar]
  8. Hatano S., Takeuchi I. 1960; ATP content in myxomycete plasmodium and its levels in relation to some external conditions.. Protoplasma 52:169–183
    [Google Scholar]
  9. Hato M., Ueda T., Kurihara K., Kobatake Y. 1976a; Changes in zeta potential and membrane potential of slime mold Physarum polycephalum in response to chemical stimuli.. Biochimica et biophysica acta 426:73–80
    [Google Scholar]
  10. Hato M., Ueda T., Kurihara K., Kobatake Y. 1976b; Phototaxis in true slime mold Physarum polycephalum.. Cell Structure and Function 1:155–164
    [Google Scholar]
  11. Ishida N., Kurihara K., Kobatake Y. 1977; Changes in adhesive properties of slime mold Physarum polycephalum accompanied with chemo-reception.. Cytobiologie 15:269–274
    [Google Scholar]
  12. Ishida N., Kurihara K., Kobatake Y. 1978; Dynamic conformational changes of surface membrane of true slime mold in response to chemoreception revealed by reactivity with chemical agents.. Comparative Biochemistry and Physiology 60:241–246
    [Google Scholar]
  13. Israel M., Lesbats B., Manaranche R., Marsal J., Mastour-Frachon P., Meunier F.M. 1977; Related changes in amount of ACh and ATP in resting and active Torpedo nerve electroplaque synapses.. Journal of Neurochemistry 28:1259–1267
    [Google Scholar]
  14. Kincaid R.L., Mansour T.E. 1978; Chemotaxis toward carbohydrates and aminoacids in Physarum polycephalum.. Experimental Cell Research 116:377–385
    [Google Scholar]
  15. Kincaid R.L., Mansour T.E. 1979; Cyclic 3ʹ,5ʹ-AMP phosphodiesterase in Physarum polycephalum. I. Chemotaxis toward inhibitors and cyclic nucleotides.. Biochimica et biophysica acta 588:332–341
    [Google Scholar]
  16. Knowles D.J.C., Carlile M.J. 1978; The chemotactic response of plasmodia of the myxomycete Physarum polycephalum to sugars and related compounds.. Journal of General Microbiology 108:17–25
    [Google Scholar]
  17. Mito Y., Kurihara K., Kobatake Y. 1980; Selective suppression of positive chemotaxis in Physarum polycephalum by treatment with rotenone or under anaerobic condition.. European Journal of Cell Biology 21:43–47
    [Google Scholar]
  18. Sakai T., Kamiya N. 1976; ATP level in the plasmodium of Physarum polycephalum.. 29th Annual Meeting of the Japan Society for Cell Biology Sendai Japan, Abstracts p. 46:
    [Google Scholar]
  19. Ueda T., Götz von Olenhusen K. 1978; Replacement of endoplasm with artificial media in plasmodial strands of Physarum polycephalum: effects on contractility and morphology.. Experimental Cell Research 116:55–62
    [Google Scholar]
  20. Ueda T., Kobatake Y. 1977a; Hydrophobicity of biosurfaces as shown by chemoreceptive thresholds in Tetrahymena, Physarum and Nitella.. Journal of Membrane Biology 34:351–368
    [Google Scholar]
  21. Ueda T., Kobatake Y. 1977b; Changes in membrane potential, zeta potential and chemotaxis of Physarum polycephalum in response to n- alcohols, n-aldehydes and n-fatty acids.. Cyto- biologie 16:16–26
    [Google Scholar]
  22. Ueda T., Kobatake Y. 1979; Spectral analysis of fluorescence of 8-anilino-l-naphthalenesulfon- ate in chemoreception with a white plasmodium of Physarum polycephalum: evidences for conformational change in chemoreceptive membrane.. Biochimica et biophysica acta 557:199–207
    [Google Scholar]
  23. Ueda T., Terayama K., Kurihara K., Kobatake Y. 1975; Threshold phenomena in chemoreception and taxis in slime mold Physarum polycephalum.. Journal of General Physiology 65:223–234
    [Google Scholar]
  24. Ueda T., Muratsugu M., Kurihara K., Kobatake Y. 1976; Chemotaxis in Physarum polycephalum: effects of chemicals on isometric tension of the plasmodial strand in relation to chemotactic movement.. Experimental Cell Research 100:337–344
    [Google Scholar]
  25. Ueda T., Götz Von Olenhusen K., Wohlfarth-Bottermann K.E. 1978; Reaction of the contractile apparatus in Physarum to injected Ca2+, ATP, ADP and 5ʹ AMP.. Cytobiologie 18:76–94
    [Google Scholar]
  26. Yoshimoto Y., Matsumura F., Sakai T., Kamiya N. 1980; Oscillations in Ca2+ and ATP effluxes coupled with tension generation in the plasmodial strand of Physarum.. Cell Motility in the Press
    [Google Scholar]
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