1887

Abstract

Mutations in the incompatibility factor of were obtained using two techniques. Both selected for the expression of -controlled functions (associated with nuclear migration during dikaryon morphogenesis) in heterokaryotic mycelia where these were blocked by having identical alleles present. The first technique selected for clamp cell fusion in a common heterokaryon, the second selected for septum dissolution in a heterokaryon following a common mating. Two mutants, one derived by each of the selection techniques, were studied in detail. In both, mutation was inseparable by recombination from the locus and resulted in self-compatibility. Mutant monokaryons had uninucleate cells and intact septa. Thus, full regulatory control of morphogenesis in the mutant monokaryon was maintained. However, both mutations promoted clamp cell fusion in dikaryons formed between the mutants and monokaryons having the progenitor allele or the mutant self allele. Therefore, mutation leads to the loss of regulation of clamp cell fusion. A combination of a mutation and an mutation () in the same nucleus gave a haploid mycelium which resembled a true dikaryon; thus, both the and morphogenetic sequences which are expressed in the dikaryon were operating. However, these dikaryons were not fertile.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-121-1-17
1980-11-01
2021-10-21
Loading full text...

Full text loading...

/deliver/fulltext/micro/121/1/mic-121-1-17.html?itemId=/content/journal/micro/10.1099/00221287-121-1-17&mimeType=html&fmt=ahah

References

  1. Casselton L.A. 1978; Dikaryon formation in the higher basidiomycetes. In The Filamentous Fungi 3 pp. 278–297 Edited by Smith J.E., Berry D.R. London: Arnold;
    [Google Scholar]
  2. Casselton L.A., Kirkham J.B. 1975; Growth and ultrastructural studies on the mitochondrial mutant of Coprinus lagopus. Archives of Microbiology 106:215–220
    [Google Scholar]
  3. Day P.R. 1960; The structure of the A mating type locus in Coprinus lagopus. Genetics 45:641–650
    [Google Scholar]
  4. Day P.R. 1963; Mutations of the A mating type factor in Coprinus lagopus. Genetical Research 4:55–64
    [Google Scholar]
  5. Esser K., Semerdzieva M., Stahl U. 1974; Genetische Untersuchungen an dem Basidio- myceten Agrocybe aegerita. 1. Eine Korrelation zwischen dem Zeitpunkt der Fruchtkorperbildung und monokaryotischem Früchten und ihre Bedeutung für Züchtung und Morphogenese. Theoretical and Applied Genetics 45:77–85
    [Google Scholar]
  6. Giesy R.M., Day P.R. 1965; The septal pores of Coprinus lagopus in relation to nuclear migration. American Journal of Botany 52:287–293
    [Google Scholar]
  7. Hoffman R.M., Raper J.R. 1974; Genetic impairment of energy conservation in development of Schizophyllum. Efficient mitochondria in energy-starved cells. Journal of General Microbiology 82:67–75
    [Google Scholar]
  8. Janszen F.H.A., Wessels J.G.H. 1970; Enzymatic dissolution of hyphal septa in a basidiomycete. Antonie van Leeuwenhoek 36:255–257
    [Google Scholar]
  9. Koltin Y. 1968; The genetic structure of the incompatibility factors of Schizophyllum commune: comparative studies of primary mutations in the B factor. Molecular and General Genetics 102:196–203
    [Google Scholar]
  10. Koltin Y., Flexer A.S. 1969; Alteration of nuclear distribution in B mutant strains of Schizophyllum commune. Journal of Cell Science 4:739–747
    [Google Scholar]
  11. Koltin Y., Raper J.R. 1966; Schizophyllum commune: new mutations in the B incompatibility factor. Science 154:510–511
    [Google Scholar]
  12. Koltin Y., Raper J.R., Simchen G. 1967; The genetic structure of the incompatibility factors of Schizophyllum commune: the B factor. Proceedings of the National Academy of Sciences of the United States of America 5755–62
    [Google Scholar]
  13. Lewis D. 1961; Genetic analysis of methionine suppressors in Coprinus. Genetical Research 2:141–155
    [Google Scholar]
  14. Niederpruem D.J. 1969; Direct studies of nuclear movements in Schizophyllum commune. Archiv für Mikrobiologie 64:387–395
    [Google Scholar]
  15. Parag Y. 1962; Mutations in the B incompatibility factor of Schizophyllum commune. Proceedings of the National Academy of Sciences of the United States of America 48743–750
    [Google Scholar]
  16. Raper J.R. 1966 Genetics of Sexuality in Higher Fungi New York: The Ronald Press Co;
    [Google Scholar]
  17. Raper J.R., Boyd D.H., Raper C.A. 1965; Primary and secondary mutations at the incompatibility loci in Schizophyllum. Proceedings of the National Academy of Sciences of the United States of America 531324–1332
    [Google Scholar]
  18. Raudaskoski M., Stamberg J., Bawnik N.E., Koltin Y. 1976; Mutational analysis of natural alleles at the B incompatibility factor of Schizophyllum commune: α2 and β6. Genetics S3:507–516
    [Google Scholar]
  19. Shahriari H., Casselton L.A. 1974; Suppression of methionine mutants in Coprinus.1. Complementation and allele specificity as criteria of suppressor gene action. Molecular and General Genetics 134:85–92
    [Google Scholar]
  20. Stahl U., Esser K. 1976; Genetics of fruit body production in higher basidiomycetes. 1. Monokaryotic fruiting and its correlation with dikaryotic fruiting in Polyporus ciliatus. Molecular and General Genetics 148:183–197
    [Google Scholar]
  21. Swiezynski K. M., Day P. R. 1960a; Heterokaryon formation in Coprinus lagopus. Genetical Research 1:114–128
    [Google Scholar]
  22. Swiezynski K. M., Day P. R. 1960b; Migration of nuclei in Coprinus lagopus. Genetical Research 1:129–139
    [Google Scholar]
  23. Van De Valk P., Wessels J.G.H. 1973; Mitotic synchrony in multinucleate Schizophyllum protoplasts. Protoplasma 78:427–432
    [Google Scholar]
  24. Wessels J.G.H. 1965; Morphogenesis and biochemical processes in Schizophyllum commune Fr. Wentia 13:1–113
    [Google Scholar]
  25. Wessels J.G.H. 1969; Biochemistry of sexual morphogenesis in Schizophyllum commune: effect of mutations affecting the incompatibility system on cell wall metabolism. Journal of Bacteriology 98:697–704
    [Google Scholar]
  26. Wessels J.G.H., Niederpruem D.J. 1967; Role of a cell wall glucan degrading enzyme in mating of Schizophyllum commune. Journal of Bacteriology 94:1594–1602
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-121-1-17
Loading
/content/journal/micro/10.1099/00221287-121-1-17
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error