1887

Abstract

In , the fatty acid moiety of the phospholipids was affected differently during growth in the presence of 1·1 -methanol or 0·7 -ethanol, though at these concentrations methanol and ethanol had the same effects on growth rate and completely inhibited sporulation. Synthesis of phosphatidylglycerol was also strongly inhibited and the amount of total cell phospholipids was reduced by 50% by both alcohols. The composition of fatty acids, especially the relative concentration of 12-methyltetradecanoic acid, was modified only by ethanol; in bacteria grown in the presence of methanol, changes in fatty acid composition were negligible. In non-sporulating mutants, synthesis of phosphatidylglycerol was much less affected than in the wild-type and synthesis of phosphatidylethanolamine was increased. In these strains, fatty acid composition was also modified by ethanol but unaffected by methanol.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-121-1-139
1980-11-01
2024-04-23
Loading full text...

Full text loading...

/deliver/fulltext/micro/121/1/mic-121-1-139.html?itemId=/content/journal/micro/10.1099/00221287-121-1-139&mimeType=html&fmt=ahah

References

  1. Bernard M.C., Brisou J., Denis F., Rosenberg A.J. 1972; Présence d’une phospholipase A chez Escherichia coli 0118.1-mise en évidence de deux états de l’enzyme et propriétés. Biochimie 54:261–270
    [Google Scholar]
  2. Bishop D.G., Rutberg L., Samuelsson B. 1967; The chemical composition of the cytoplasmic membrane of Bacillus subtilis. European Journal of Biochemistry 2:448–453
    [Google Scholar]
  3. Bligh E.G., Dyer W.J. 1959; A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology 37:911–917
    [Google Scholar]
  4. Bohin J.B., Rigomier D., schaeffer P. 1976; Ethanol sensitivity of sporulation in Bacillus subtilis: a new tool for the analysis of the sporulation process. Journal of Bacteriology 127:934–940
    [Google Scholar]
  5. Buttke T.M., Ingram L.O. 1978; Mechanism of ethanol induced changes in lipid composition of Escherichia coli: inhibition of saturated fatty acid synthesis in vivo. Biochemistry 17:637–644
    [Google Scholar]
  6. Fried V.A., Novick A. 1975; Organic solvents as probes for the structure and function of bacterial membrane: effects of ethanol on the wild type and an ethanol-resistant mutant of Escherichia coli K12. Journal of Bacteriology 114:239–248
    [Google Scholar]
  7. Fujii D.K., Fulco A.J. 1977; Biosynthesis of unsaturated fatty acids. Hyperinduction and modulation of desaturase synthesis. Journal of Biological Chemistry 252:3660–3670
    [Google Scholar]
  8. Gill C.O. 1976; Effects of growth temperature on the lipids of Pseudomonas fluorescens. Journal of General Microbiology 89:293–298
    [Google Scholar]
  9. Harold F.M. 1970; Antimicrobial agents and membrane function. Advances in Microbial Physiology 4:45–104
    [Google Scholar]
  10. Hui F.K., Barton P.G. 1973; Mesomorphic behaviour of some phospholipids with aliphatic alcohols and other nonionic substances. Bio- chimica et biophysica acta 296:510–517
    [Google Scholar]
  11. Ingram L.O. 1977a; Preferential inhibition of phosphatidylethanolamine synthesis in Escherichia coli by alcohols. Canadian Journal of Microbiology 23:779–789
    [Google Scholar]
  12. Ingram L.O. 1977b; Changes in lipid composition of Escherichia coli resulting from growth with organic solvents and with food additives. Applied and Environmental Microbiology 33:1233–1236
    [Google Scholar]
  13. Van Iterson W., Op Den Kamp J.A.F. 1969; Bacterial shaped gymnoplasts (protoplasts) of Bacillus subtilis. Journal of Bacteriology 99:304–315
    [Google Scholar]
  14. Jain M.K., Wu N.M. 1977; Effect of small molecules on the dipalmitoyl lecithin liposomal bilayers: phase transition in lipid bilayer. Journal of Membrane Biology 34:157–201
    [Google Scholar]
  15. Kates M., Kushner D.J., James A.T. 1962; The lipid composition of Bacillus cereus as influenced by the presence of alcohols in the culture medium. Canadian Journal of Biochemistry and Physiology 40:83–93
    [Google Scholar]
  16. Lenaz G., Bertoli E., Curatola G., Mazzanti L., Bigi A. 1976; Lipid protein interactions in mitochondria: spin and fluorescence probe studies on the effects of n-alkanols on phospholipid vesicles and on mitochondrial membranes. Archives of Biochemistry and Biophysics 172:278–298
    [Google Scholar]
  17. Marr A.G., Ingraham J.L. 1962; Effect of temperature on the composition of fatty acids in Escherichia coli. Journal of Bacteriology 84:1261–1267
    [Google Scholar]
  18. Mcintyre T.M., Chamberlain B.K., Webster R.E., Bell R.M. 1977; Mutants of Escherichia coli defective in membrane phospholipid synthesis. Effects of cessation and reinitiation of phospholipid synthesis on macromolecular synthesis and phospholipid turnover. Journal of Biological Chemistry 252:4487–4493
    [Google Scholar]
  19. Merlie J.P., Pizer L.T. 1973; Regulation of phospholipid synthesis in Escherichia coli by guanosine tetraphosphate. Journal of Bacteriology 116:355–366
    [Google Scholar]
  20. Michaelson D.M., Horwitz A.F., Klein M.P. 1974; Head group modulation of membrane fluidity in sonicated phospholipid dispersions. Biochemistry 13:2605–2611
    [Google Scholar]
  21. Michel J.F., Cami B., schaeffer P. 1968; Selection de mutants de Bacillus subtilis bloques au début de la sporulation. Annales de l’lnstitut Pasteur 114:11–27
    [Google Scholar]
  22. Munro G.F., Bell C.A. 1973; Effects of external osmolarity on phospholipid metabolism in E. coli B. Journal of Bacteriology 116:257–262
    [Google Scholar]
  23. Okuyama H., Yamada K., Kameyama Y., Ikezawa H., Akamatsu Y., Nojima S. 1977; Regulation of membrane lipid synthesis in Escherichia coli after shifts in temperature. Biochemistry 16:2668–2673
    [Google Scholar]
  24. Patterson S.J., Butler K.W., Huang P., Labelle J., Smith I.C.P., schneider H. 1972; The effects of alcohols on lipid bilayers: a spin label study. Biochimica et biophysica acta 246:1062–1072
    [Google Scholar]
  25. Rigomier D., Lubochinsky B. 1974; Méta- bolisme des phospholipides chez des mutants asporogènes de Bacillus subtilis au cours de la croissance exponentielle. Annales de Micro- biologie 125B:295–303
    [Google Scholar]
  26. Rigomier D., Lubochinsky B., schaeffer P. 1974; Composition en phospholipides de mutants asporogènes de Bacillus subtilis. Comptes rendus hebdomadaires des seances de l’Academie des sciences 278:2059–2062
    [Google Scholar]
  27. Saito Y., Mcelhaney R.N. 1977; Membrane lipid biosynthesis in Acholeplasma laidlawii B: incorporation of exogenous fatty acids into membrane glyco- and phospholipids by growing cells. Journal of Bacteriology 132:485–496
    [Google Scholar]
  28. Sandermann H. 1978; Regulation of membrane enzymes by lipids. Biochimica et biophysica acta 515:209–237
    [Google Scholar]
  29. scandella C.J., Kornberg A. 1969; Biochemical studies of bacterial sporulation and germination. XV. Fatty acids in growth, sporula- tion and germination of Bacillus megaterium. Journal of Bacteriology 98:82–86
    [Google Scholar]
  30. Sinensky M. 1971; Temperature control of phospholipid biosynthesis in Escherichia coli. Journal of Bacteriology 106:449–455
    [Google Scholar]
  31. Sinensky M. 1974; Homeoviscous adaption - an homeostatic process that regulates the viscosity of the membrane lipids in Escherichia coli. Proceedings of the National Academy of sciences of the United States of America 71:522–525
    [Google Scholar]
  32. Vaughan D.J., Keough K.M. 1974; Changes in phase transitions of phosphatidylethanolamine - and phosphatidylcholine - water dispersions induced by small modifications in the headgroup and backbone regions. FEBS Letters 47:158–161
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-121-1-139
Loading
/content/journal/micro/10.1099/00221287-121-1-139
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error