@article{mbs:/content/journal/micro/10.1099/00221287-120-2-545, author = "Murata, Kousaku and Tani, Keiko and Kato, Jyoji and Chibata, Ichiro", title = "Excretion of Glutathione by Methylglyoxal-resistant Escherichia coli", journal= "Microbiology", year = "1980", volume = "120", number = "2", pages = "545-547", doi = "https://doi.org/10.1099/00221287-120-2-545", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-120-2-545", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "A methylglyoxal-resistant mutant of Escherichia coli B excreted glutathione into the growth medium, especially during growth on medium containing methylglyoxal. In the presence of methylglyoxal, the total amount of glutathione excreted was increased about 50-fold over that of the wild-type strain. The resistant mutant had high activities of two enzyme systems: a glutathione-forming enzyme system (consisting of γ-glutamylcysteine synthetase and glutathione synthetase) and a glyoxalase system (consisting of glyoxalase I and glyoxalase II). Methylglyoxal resistance appeared to be due to the simultaneous increase in the activities of these two enzyme systems.", }