1887

Abstract

A methylglyoxal-resistant mutant of B excreted glutathione into the growth medium, especially during growth on medium containing methylglyoxal. In the presence of methylglyoxal, the total amount of glutathione excreted was increased about 50-fold over that of the wild-type strain. The resistant mutant had high activities of two enzyme systems: a glutathione-forming enzyme system (consisting of -glutamylcysteine synthetase and glutathione synthetase) and a glyoxalase system (consisting of glyoxalase I and glyoxalase II). Methylglyoxal resistance appeared to be due to the simultaneous increase in the activities of these two enzyme systems.

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/content/journal/micro/10.1099/00221287-120-2-545
1980-10-01
2024-12-05
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References

  1. Ackerman R. S., Cozzarelli N. R., Epstein W. 1974; Accumulation of toxic concentrations of methylglyoxal by wild-type Escherichia coli K-12. Journal of Bacteriology 119:357–362
    [Google Scholar]
  2. Adelberg E. A., Mandel M., Chen G. C. C. 1965; Optimal conditions for mutagenesis by N-methyl-N´-nitro-N-nitrosoguanidine in Escherichia coli. . Biochemical and Biophysical Research Communications 18:788–795
    [Google Scholar]
  3. Cooper R. A., Anderson A. 1970; The formation and catabolism of methylglyoxal during glycolysis in Escherichia coli. . FEBS Letters 11:273–276
    [Google Scholar]
  4. Freedberg W. B., Kistler W. S., Lin E. C. C. 1971; Lethal synthesis of methylglyoxal by Escherichia coli during unregulated glycerol metabolism. Journal of Bacteriology 108:137–144
    [Google Scholar]
  5. Hopper D. J., Cooper R. A. 1972; The purification and properties of Escherichia coli methylglyoxal synthase. Biochemical Journal 128:321–329
    [Google Scholar]
  6. Krymkiewicz N., Dieguez E., Pekarte U. D., Zwaig N. 1971; Properties and mode of action of bactericidal compound ( = methylglyoxal) produced by a mutant of Escherichia coli. . Journal of Bacteriology 108:1338–1347
    [Google Scholar]
  7. Murata K., Tani K., Kato J., Chibata 1. 1979; Application of immobilized ATP in the production of glutathione by a multi-enzyme system. Journal of Applied Biochemistry 1:283–290
    [Google Scholar]
  8. Szent-Györgyi A. 1968 Bioelectronics, A Study in Cellular Regulations, Defense and Cancer New York: Academic Press;
    [Google Scholar]
  9. Tietze F. 1969; Enzymic method for quantitative determination of nanogram amounts of total and oxidized glutathione. Analytical Biochemistry 27:502–522
    [Google Scholar]
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