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Cytochrome P-450 was measured in whole cell suspensions of various yeasts, with Saccharomyces cerevisiae and S. uvarum (formerly carlsbergensis) serving as positive control species. Eleven yeasts, when grown on a medium with glucose (50 g 1−1) as carbon and energy source, contained cytochrome P-450. These were the Ascomycotina Debaryomyces hansenii, Hansenula anomala, Kluyveromyces fragilis, Pichia fermentans, Saccharomyces bayanus, S. chevalieri, S. italicus and Schizosaccharomyces japonicus, and the Deutero-mycotina Brettanomyces anomalus, Torulopsis dattila and T. glabrata. The amount of cytochrome P-450 varied 60- to 70-fold between the species. The position of the Soret band in reduced CO spectra also differed from one species to another. Cytochrome P-450 could not be detected in either Candida utilis or a Rhodotorula sp.
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