Summary: Intracellular concentrations of ammonia and amino acids, and the specific activity of the acidic amino acid transport system responsible for transport of low concentrations of glutamate in have been measured during germination on glutamate and ammonia and during various subsequent incubations of the germinated conidia. It is concluded that intracellular glutamine (and not ammonia) is involved in repression of synthesis of the transport system for low concentrations of glutamate and that intracellular ammonia (but not glutamine) is able to inhibit the preformed permease. This conclusion supports our suggestion that glutamine synthetase may well have a more significant role in the regulation of some aspects of nitrogen metabolism than does glutamate dehydrogenase as was previously proposed (Pateman , 1973).


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