1887

Abstract

Intracellular concentrations of ammonia and amino acids, and the specific activity of the acidic amino acid transport system responsible for transport of low concentrations of glutamate in have been measured during germination on glutamate and ammonia and during various subsequent incubations of the germinated conidia. It is concluded that intracellular glutamine (and not ammonia) is involved in repression of synthesis of the transport system for low concentrations of glutamate and that intracellular ammonia (but not glutamine) is able to inhibit the preformed permease. This conclusion supports our suggestion that glutamine synthetase may well have a more significant role in the regulation of some aspects of nitrogen metabolism than does glutamate dehydrogenase as was previously proposed ( Pateman , 1973 ).

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-120-2-447
1980-10-01
2021-07-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/120/2/mic-120-2-447.html?itemId=/content/journal/micro/10.1099/00221287-120-2-447&mimeType=html&fmt=ahah

References

  1. Cook R. J., Anthony C. 1978a; The ammonia and methylamine active transport system of Aspergillus nidulans. Journal of General Microbiology 109:265–274
    [Google Scholar]
  2. Cook R. J., Anthony C. 1978b; Regulation by glutamine of ammonia transport in Aspergillus nidulans. Journal of General Microbiology 109:275–286
    [Google Scholar]
  3. Hofstee B. H. J. 1952; On the evaluation of the constants Vm and Km in enzyme reactions. Science 116:329–331
    [Google Scholar]
  4. Meyers E., Knight S. G. 1953; Studies on the intracellular amino acids of Penicillium roqueforti. Mycologia 53:115–122
    [Google Scholar]
  5. Pateman J. A., Kinghorn J. R., Dunn E., Forbes E. 1973; Ammonium regulation in Aspergillus nidulans. Journal of Bacteriology 114:943–950
    [Google Scholar]
  6. Pateman J. A., Kinghorn J. R., Dunn E. 1974; Regulatory aspects of L-glutamate transport inAspergillus nidulans. Journal of Bacteriology 119:534–542
    [Google Scholar]
  7. Kessler C., Koga T. 1971; α-Cyanoamino acids and related nitriles as inhibitors of glutamate decarboxylase. Biochimica et biophysica acta 242:473–483
    [Google Scholar]
  8. Robinson J. H., Anthony C., Drabble W. T. 1973a; The acidic amino acid permease of Aspergillus nidulans. Journal of General Microbiology 79:53–63
    [Google Scholar]
  9. Robinson J. H., Anthony C., Drabble W. T. 1973b; Regulation of the acidic amino acid permease of Aspergillus nidulans. Journal of General Microbiology 79:65–80
    [Google Scholar]
  10. Robinson J. H., Anthony C., Drabble W. T. 1974; The utilization of nitrogen sources by Aspergillus clavatus. Journal of General Microbiology 85:23–28
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-120-2-447
Loading
/content/journal/micro/10.1099/00221287-120-2-447
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error