RT Journal Article SR Electronic(1) A1 Brewin, N. J. A1 Beringer, J. E. A1 Johnston, A. W. B.YR 1980 T1 Plasmid-mediated Transfer of Host-range Specificity Between Two Strains of Rhizobium leguminosarum JF Microbiology, VO 120 IS 2 SP 413 OP 420 DO https://doi.org/10.1099/00221287-120-2-413 PB Microbiology Society, SN 1465-2080, AB Transfer of nodulation ability (Nod+) from derivatives of Rhizobium leguminosarum strain TOM to strain 16015, a non-nodulating mutant of strain 300, occurred at frequencies of 10−5 to 10−6 per recipient. All 39 Nod+ derivatives of strain 16015 examined had the host-range specificity of the donor strain TOM, i.e. the ability to nodulate the pea cultivar ‘Afghanistan’ and the primitive pea line JI 241 in addition to the commercial variety ‘Wisconsin Perfection’ which can be nodulated by strain 300. The transfer of Nod+ was associated with the appearance in strain 16015 of a new plasmid band on agarose gels (mol. wt about 160 × 106) which corresponded in size to one of the three plasmid bands found in strain TOM: we term this new plasmid pRL5JI. In addition to genes for Nod+ and host-range, the plasmid pRL5JI carries genes for nodule function (Fix+) that are absent from strain 16015. Although strain TOM produces a medium-sized bacteriocin, this property was not co-transferred with pRL5JI. The introduction of pRL5JI did not result in the elimination of any of the resident plasmids of strain 16015. The plasmid pRL5JI differs in many respects from the transmissible bacteriocinogenic plasmids pRL1JI, pRL3JI and pRL4JI previously found, and it appears to belong to a different incompatibility group., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-120-2-413