1887

Abstract

Transfer of nodulation ability (Nod) from derivatives of strain TOM to strain 16015, a non-nodulating mutant of strain 300, occurred at frequencies of 10 to 10 per recipient. All 39 Nod derivatives of strain 16015 examined had the host-range specificity of the donor strain TOM, i.e. the ability to nodulate the pea cultivar ‘Afghanistan’ and the primitive pea line JI 241 in addition to the commercial variety ‘Wisconsin Perfection’ which can be nodulated by strain 300. The transfer of Nod was associated with the appearance in strain 16015 of a new plasmid band on agarose gels (mol. wt about 160 × 10) which corresponded in size to one of the three plasmid bands found in strain TOM: we term this new plasmid pRL5JI. In addition to genes for Nod and host-range, the plasmid pRL5JI carries genes for nodule function (Fix) that are absent from strain 16015. Although strain TOM produces a -sized bacteriocin, this property was not co-transferred with pRL5JI. The introduction of pRL5JI did not result in the elimination of any of the resident plasmids of strain 16015. The plasmid pRL5JI differs in many respects from the transmissible bacteriocinogenic plasmids pRL1JI, pRL3JI and pRL4JI previously found, and it appears to belong to a different incompatibility group.

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1980-10-01
2021-05-12
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References

  1. Beringer J. E. 1974; R factor transfer in Rhiz- obium leguminosarum. . Journal of General Microbiology $ 4:188–198
    [Google Scholar]
  2. Beringer J. E., Hoggan S. A., Johnston A. W. B. 1978; Linkage mapping in Rhizobium leguminosarum by means of R plasmid-mediated recombination. Journal of General Microbiology 104:201–207
    [Google Scholar]
  3. Brewin N. J., Beringer J. E., Buchanan-Wol-Laston A. V., Johnston A. W. B., Hirsch P. R. 1980; Transfer of symbiotic genes with bacteriocinogenic plasmids in Rhizobium leguminosarum. . Journal of General Microbiology 116:261–270
    [Google Scholar]
  4. Buchanan-Wollaston A. V., Beringer J. E., Brewin N. J., Hirsch P. R., Johnston A. W. B. 1980; Isolation of symbiotically defective mutants of Rhizobium leguminosarum by insertion of the transposon Tn5 into a transmissible plasmid. Molecular and General Genetics 178:185–190
    [Google Scholar]
  5. Casse F., Boucher C., Julliot J. S., Michel M., Dénarié J. 1979; Identification and characterization of large plasmids in Rhizobium meliloti using agarose gel electrophoresis. Journal of General Microbiology 113:229–242
    [Google Scholar]
  6. Gross D. C., Vidaver A. K., Klucas R. V. 1979; Plasmids, biological properties and efficacy of nitrogen fixation in Rhizobium japoni- cum strains indigenous to alkaline soils. Journal of General Microbiology 114:257–266
    [Google Scholar]
  7. Haas D., Holloway B. W. 1976; R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa. . Molecular and General Genetics 144:243–251
    [Google Scholar]
  8. Hirsch P. R. 1979; Plasmid-determined bact- eriocin production by Rhizobium leguminosarum. . Journal of General Microbiology 113:219–228
    [Google Scholar]
  9. Hirsch P. R., Van Montagu M., Johnston A. W. B., Brewin N. J., Schell J. 1980; Physical identification of bacteriocinogenic, nodu- lation and other plasmids in strains of Rhizobium leguminosarum. . Journal of General Microbiology 120:403–412
    [Google Scholar]
  10. Holl F. B. 1975; Host plant control of the inheritance of dinitrogen fixation in the Pisum- Rhizobium symbiosis. Euphytica 24:767–770
    [Google Scholar]
  11. Johnston A. W. B., Beringer J. E. 1975; Identification of the Rhizobium strains in pea root nodules using genetic markers. Journal of General Microbiology 87:343–350
    [Google Scholar]
  12. Johnston A. W. B., Beringer J. E. 1976; Pea root nodules containing more than one Rhizobium species. Nature; London: 263502–504
    [Google Scholar]
  13. Johnston A. W. B., Beynon J. L., Buchanan-Wollaston A. V., Setchell S. M., Hirsch P. R., Beringer J. E. 1978; High frequency transfer of nodulating ability between strains and species of Rhizobium. . Nature; London: 276635–636
    [Google Scholar]
  14. Josey D. P., Beynon J. L., Johnston A. W. B., Beringer J. E. 1979; Strain identification in Rhizobium using intrinsic antibiotic resistance. Journal of Applied Bacteriology 46:343–350
    [Google Scholar]
  15. Lie T. A., Hille D., Lambers R., Houwers A. 1976; Symbiotic specialisation in pea plants: some environmental effects on nodulation and nitrogen fixation. In Symbiotic Nitrogen Fixation in Plants pp. 319–333 Nutman P. S. Edited by Cambridge: Cambridge University Press;
    [Google Scholar]
  16. Loper J. E., Kado C. E. 1979; Host range conferred by the virulence-specifying plasmid of Agrobacterium tumefaciens. . Journal of Bacteriology 139:591–596
    [Google Scholar]
  17. Van Montagu M., Schell J. 1979; The plasmids of Agrobacterium tumefaciens. . In Plasmids of Medical, Environmental and Commercial Importance pp. 71–95 Timmis K. N., Pühler A. Edited by Amsterdam: Elsevier/ North Holland;
    [Google Scholar]
  18. Moore L., Warren G., Strobel G. 1979; Involvement of a plasmid in the hairy root disease of plants caused by Agrobacterium rhizo-genes. . Plasmid 2:617–626
    [Google Scholar]
  19. Nuti M. P., Lepidi A. A., Prakash R. K., Schilperoort R. A., Cannon F. C. 1979; Evidence for nitrogen fixation (nif) genes on indigenous Rhizobium plasmids. Nature; London: 282533–535
    [Google Scholar]
  20. Thomashow M. F., Panagopoulos C. G., Gordon M. P., Nester E. W. 1980; Host range of Agrobacterium tumefaciens is determined by the Ti plasmid. Nature; London: 283794–796
    [Google Scholar]
  21. Vincent J. M. 1970 A Manual for the Practical Study of the Root Nodule Bacteria. Oxford: Blackwell Scientific Publications;
    [Google Scholar]
  22. Winarno R., Lie T. A. 1979; Competition between Rhizobium strains in nodule formation: interactions between nodulating and non-nodu- lating strains. Plant and Soil 51:135–142
    [Google Scholar]
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