1887

Abstract

Four independent mutants of PAO lacking the activity of the pyruvate dehydrogenase complex have been isolated. They resembled mutants of and in requiring acetate as an essential supplement for aerobic growth on glucose, succinate or lactate and in their ability to utilize acetate as sole carbon and energy source. Assays for the individual components of the pyruvate dehydrogenase complex indicated that they lacked the pyruvate dehydrogenase component (E1) or the pyruvate dehydrogenase and dihydrolipoamide acetyltransferase components (E1 and E2) but not the lipoamide dehydrogenase component (E3). Genetic studies with plasmid R68.45-mediated conjugation and phage F116L-mediated transduction indicated that the mutations are located at approximately 15 min in the PAO linkage map.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-120-2-385
1980-10-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/120/2/mic-120-2-385.html?itemId=/content/journal/micro/10.1099/00221287-120-2-385&mimeType=html&fmt=ahah

References

  1. Bridger W. A., Ramaley R. F., Boyer P. D. 1969; Succinyl coenzymeA synthetase from Escherichia coli. . Methods in Enzymology 13:70–75
    [Google Scholar]
  2. Creaghan I. T., Guest J. R. 1972; Amber mutants of the α-ketoglutarate dehydrogenase gene of Escherichia coli K12. Journal of General Microbiology 71:207–220
    [Google Scholar]
  3. De Abreu R. A., De Kok A., Veeger C. 1977; Transformation of the 4-component pyruvate dehydrogenase from Azotobacter vinelandii into a 3-component complex. FEBS Letters 82:89–92
    [Google Scholar]
  4. Fothergill J. C., Guest J. R. 1977; Catabolism of l-lysine by Pseudomonas aeruginosa. . Journal of General Microbiology 99:139–155
    [Google Scholar]
  5. Freese E., Fortnagel U. 1969; Growth and sporulation of Bacillus subtilis mutants blocked in the pyruvate dehydrogenase complex. Journal of Bacteriology 99:745–756
    [Google Scholar]
  6. Guest J. R. 1974; Gene-protein relationships of the α-keto acid dehydrogenase complexes of Escherichia coli K12: chromosomal location of the lipoamide dehydrogenase gene. Journal of General Microbiology 80:523–532
    [Google Scholar]
  7. Guest J. R. 1978; Aspects of the molecular biology of lipoamide dehydrogenase. Advances in Neurology 21:219–244
    [Google Scholar]
  8. Guest J. R., Creaghan I. T. 1973; Gene-protein relationships of the α-keto acid dehydrogenase complexes of Escherichia coli K12: isolation and characterization of lipoamide dehydrogenase mutants. Journal of General Microbiology 75:197–210
    [Google Scholar]
  9. Guest J. R., Creaghan I. T. 1974; Further studies with lipoamide dehydrogenase mutants of Escherichia coli K12. Journal of General Microbiology 81:237–245
    [Google Scholar]
  10. Haas D., Holloway B. W. 1976; R factor variants with enhanced sex factor activity in Pseudomonas aeruginosa. . Molecular and General Genetics 144:243–251
    [Google Scholar]
  11. Haas D., Holloway B. W., Schambøck A., Leisinger T. 1977; The genetic organization of arginine biosynthesis in Pseudomonas aeruginosa. . Molecular and General Genetics 154:7–22
    [Google Scholar]
  12. Hager L. P., Kornberg H. L. 1961; On the mechanism of α-oxoglutarate oxidation in Escherichia coli. . Biochemical Journal 78:194–198
    [Google Scholar]
  13. Henderson C. E., Perham R. N., Finch J. T. 1979; Structure and symmetry of Bacillus stearothermophilus pyruvate dehydrogenase multienzyme complex and implications for eucaryote evolution. Cell 17:85–93
    [Google Scholar]
  14. Henning U., Dennert G., Hertel R., Shipp W. S. 1966; Translation of the structural gene of the E. coli pyruvate dehydrogenase complex. Cold Spring Harbor Symposia on Quantitative Biology 31:227–234
    [Google Scholar]
  15. Hoch J. A., Coukoulis H. J. 1978; Genetics of the a-ketoglutarate dehydrogenase complex of Bacillus subtilis. . Journal of Bacteriology 133:265–269
    [Google Scholar]
  16. Holloway B. W., Krishnapillai V., Morgan A. F. 1979; Chromosomal genetics of Pseudomonas. . Microbiological Reviews 43:73–102
    [Google Scholar]
  17. Knight E., Gunsalus I. C. 1962; Formation and breakdown of acyl lipoic acids. Methods in Enzymology 5:651–656
    [Google Scholar]
  18. Langley D., Guest J. R. 1974; Biochemical and genetic characteristics of deletion and other mutant strains of Salmonella typhimurium LT2 lacking α-keto acid dehydrogenase complex activities. Journal of General Microbiology 82:319–335
    [Google Scholar]
  19. Lipmann F., Tuttle L. C. 1945; A specific micromethod for the determination of acyl phosphates. Journal of Biological Chemistry 159:21–28
    [Google Scholar]
  20. Payton M. A., McCullough W., Roberts C. F., Guest J. R. 1977; Two unlinked genes for the pyruvate dehydrogenase complex in Aspergillus nidulans. . Journal of Bacteriology 129:1222–1226
    [Google Scholar]
  21. Reed L. J. 1974; Multienzyme complexes. Accounts of Chemical Research 7:40–46
    [Google Scholar]
  22. Stanisich V. A., Holloway B. W. 1972; A mutant sex factor of Pseudomonas aeruginosa. . Genetical Research 19:91–108
    [Google Scholar]
  23. Watson J. M., Holloway B. W. 1978; Chromosome mapping in Pseudomonas aeruginosaPAT. Journal of Bacteriology 133:1113–1125
    [Google Scholar]
  24. Willms C. R., Oliver R. M., Henney H. R., Mukherjee B. B., Reed L. J. 1967; α-Keto-acid dehydrogenase complexes. VI. Dissociation and reconstitution of the dihydrolipoyl trans- acetylase of E. coli. . Journal of Biological Chemistry 242:889–897
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-120-2-385
Loading
/content/journal/micro/10.1099/00221287-120-2-385
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error