A micro-organism capable of growth on cyclohexane as the sole carbon source has been isolated from soil of an ash wood; the organism has been identified as a pseudomonad. Growth, respiration and enzymic studies with the organism are consistent with an oxidation route for cyclohexane proceeding via cyclohexanol, cyclohexanone, ε-caprolactone, 6-hydroxycaproate and adipate. Cell-free extracts of the organism grown on cyclohexane demonstrated cyclohexane hydroxylase activity which was found to be very labile, dependent on molecular oxygen and specific for NADH; the product of this reaction was identified as cyclohexanol.


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