1887

Abstract

The activity of enzymes involved in polyol metabolism was investigated in uredospores and mycelial cultures of the flax rust fungus, Mycelial cultures displayed polyol dehydrogenase activity with a range of sugars using either NADH or NADPH as a cofactor. Highest activity was found with fructose and NADPH. The relative pattern with different substrates was unaffected by the carbohydrate used in the culture medium. Highest absolute activity was detected with culture media containing -glucitol or -mannitol. Mannitol and -arabitol were the polyols oxidized by mycelial extracts. Flax rust uredospores could use aldohexoses, ketohexose and aldopentoses as substrates for polyol synthesis. Uredospore extracts were also capable of oxidizing -arabitol with NAD as cofactor.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-120-1-85
1980-09-01
2021-05-14
Loading full text...

Full text loading...

/deliver/fulltext/micro/120/1/mic-120-1-85.html?itemId=/content/journal/micro/10.1099/00221287-120-1-85&mimeType=html&fmt=ahah

References

  1. Adomako D., Kaye M.A.G., Lewis D. H. 1972; Carbohydrate metabolism in Chaetomium globosum. III. The metabolism of mannitol. New Phytologist 71:467–476
    [Google Scholar]
  2. Birken S., Pisano M. A. 1976; Purification and properties of a polyol dehydrogenase from Cephalosporium chrysogenum. Journal of Bacteriology 125:225–232
    [Google Scholar]
  3. Coffey M. D., Allen P. J. 1973; Nutrition of Melampsora lini and Puccinia helianthi. Transactions of the British Mycological Society 60:245–260
    [Google Scholar]
  4. Daly J. M., Inman R. E., Livne A. 1962; Carbohydrate metabolism in higher plant tissues infected with obligate parasites. Plant Physiology 37:531–538
    [Google Scholar]
  5. Edmundowicz J. M., Wriston J. C. 1963; Mannitol dehydrogenase from Agaricus campes-tris. Journal of Biological Chemistry 238:3539–3541
    [Google Scholar]
  6. Hendrix J. W., Daly J. M., Livne A. 1964; Pyridine nucleotide-linked enzymatic reduction of triose, pentose and hexose phosphates associated with rust infections. Phytopathology 54:895 (abstract).
    [Google Scholar]
  7. Hers H. G. 1960; Le méchanisme de la formation du fructose séminal et du fructose foetal. Bio-chimica et biophysica acta 37:127–138
    [Google Scholar]
  8. Holligan P. M., Jennings D. H. 1972; Carbohydrate metabolism in the fungus Dendryphiella salina. II. The influence of different carbon and nitrogen sources on the accumulation of mannitol and arabitol. New Phytologist 71:583–594
    [Google Scholar]
  9. Horikoshi K., Iida S., Ikeda Y. 1965; Mannitol and mannitol dehydrogenase in conidia of Aspergillus oryzae. Journal of Bacteriology 89:326–330
    [Google Scholar]
  10. Maclean D. J. 1971 Studies on axenic cultures of the wheat stem rust fungus. Ph.D. thesis; University of Queensland, Australia.:
    [Google Scholar]
  11. Maclean D. J., Scott K. J. 1976; Identification of glucitol (sorbitol) and ribitol in a rust fungus, Puccinia granmis f.sp.tritici. Journal of General Microbiology 97:83–89
    [Google Scholar]
  12. Mitchell D., Shaw M. 1968; Metabolism of glucose-14C, pyruvate-14C and mannitoI-14C by Melampsora lini. II. Conversion to soluble products. Canadian Journal of Botany 46:453–460
    [Google Scholar]
  13. Ruffner H. P., Rast D., Tobler H., Karesch H. 1978; Purification and properties of mannitol dehydrogenase from Agaricus bisporus sporo-carps. Phytochemistry 17:865–868
    [Google Scholar]
  14. Shu P., Neish A. C., Ledingham G. A. 1956; Utilization of added substrates by uredospores of wheat stem rust. Canadian Journal of Microbiology 2:559–563
    [Google Scholar]
  15. Strobel G. A., Kosuge T. 1965; Polyol metabolism in Diplodia viticola Desm. Archives of Biochemistry and Biophysics 109:622–626
    [Google Scholar]
  16. Touster O., Shaw D.R.D. 1962; Biochemistry of the acyclic polyols. Physiological Reviews 42:181–225
    [Google Scholar]
  17. Wynn W. K. 1966; NAD- and NADP-linked dehydrogenases from bean rust. Plant Physiology 41:xxvi (abstract).
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-120-1-85
Loading
/content/journal/micro/10.1099/00221287-120-1-85
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error