Schizosaccharomyces pombe grows and multiplies for many generations in the presence of chloramphenicol (CAP, 2 mg ml-1). CAP-grown cells exhibit nearly fully suppressed respiration, while fermentation occurs at the same rate as in untreated controls. The growth rate of the cultures is reduced in proportion to the diminished energy production, but the content of protein and bulk RNA per cell remain as in untreated cells. The effects of CAP are reversible. It is argued that CAP in S. pombe specifically inhibits mitochondrial protein synthesis without interfering with cytoplasmic protein synthesis.
CAP-treated cultures can be division-synchronized either by a selection technique or by a controlled temperature regimen and the synchronous divisions studied after removal of CAP.
HamburgerK.,
KramhøftB.,
NissenS.B.,
ZeuthenE.1977; Linear increase in glycolytic activity through the cell cycle of Schizosaccharomyces pombe.
. Journal of Cell Science 24:69–79
KramhøftB.,
ZeuthenE.1971; Synchronization of cell division in the fission yeast Schizosaccharomyces pombe using heat shocks. Comptes Rendus des Traveaux du Laboratoire Carlsberg 38:351–368
KramhøftB.,
HamburgerK.,
NissenS.B.,
ZeuthenE.1978; The cell cycle and glycolytic activity of Schizosaccharomyces pombe synchronized in defined medium. Carlsberg Research Communications 43:227–239
PolanshekM.M.1977; Effects of heat shock and cycloheximide on growth and division of the fission yeast Schizosaccharomyces pombe.
. Journal of Cell Science 23:1–23