1887

Abstract

Evidence was sought for gene-specific negative regulatory element of similar to the repressor/operator systems that control some operons in rpkaryotes and their viruses. Mutants of that constitutively express the stage specific enzyme α-mannosidase-2 could provide such evidence. Reconstruction experiments demonstrated that the screening technique developed would detect mutants of that type. Over 2 × 10 surviovors of heavily mutagenized cell populations were grown and their progeny tested. No mutants of the desired type were obtained. The possible implications of this finding are discussed.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-120-1-273
1980-09-01
2021-07-31
Loading full text...

Full text loading...

/deliver/fulltext/micro/120/1/mic-120-1-273.html?itemId=/content/journal/micro/10.1099/00221287-120-1-273&mimeType=html&fmt=ahah

References

  1. Alton T., Lodish H. 1977; Developmental changes in mRNA and protein synthesis in Dictyostelium discoideum. . Developmental Biology 60:180–206
    [Google Scholar]
  2. Beckwith J.R., Zipser D. 1970 The Lactose Operon New York: Cold Spring Harbor Press;
    [Google Scholar]
  3. Brenner M., Tisdale D., Loomis W.F. 1975; Techniques for rapid biochemical screening of large numbers of cell clones. Experimental Cell Research 90:249–252
    [Google Scholar]
  4. Brenner M., Dimond R.L., Loomis W.F. 1976; Rapid biochemical screening of large numbers of animal cell clones. Methods in Cell Biology 14:187–193
    [Google Scholar]
  5. Dimond R.L., Loomis W.F. 1976; Structure and function of β-glucosidases in Dictyostelium discoideum. . Journal of Biological Chemistry 251:2680–2687
    [Google Scholar]
  6. Dimond R.L., Brenner M., Loomis W.F. 1973; Mutations affecting N-acetylglucosamini- dase in Dictyostelium discoideum. . Proceedings of the National Academy of Sciences of the United States of America 703356–3360
    [Google Scholar]
  7. Free S.J., Loomis W.F. 1974; Isolation of mutations in Dictyostelium discoideum affecting α-mannosidase. Biochemie 56:1525–1528
    [Google Scholar]
  8. Free S.J., Cockburn A., Loomis W.F. 1976a; α-Mannosidase-2: a developmentally regulated enzyme in Dictyostelium discoideum. . Developmental Biology 49:539–543
    [Google Scholar]
  9. Free S.J., Schimke R.T., Loomis W.F. 1976b; Genetic analysis of the gene for a- mannosidase in Dictyostelium discoideum. . Genetics 84:159–174
    [Google Scholar]
  10. Lewin B. 1975 Gene Expression I pp. 288–290 New York: John Wiley;
    [Google Scholar]
  11. Loomis W.F. 1970; Developmental regulation of α-mannosidase in Dictyostelium discoideum. . Journal of Bacteriology 103:375–381
    [Google Scholar]
  12. Loomis W.F. 1971; Sensitivity of Dictyostelium discoideum to nucleic acid analogues. Experimental Cell Research 64:484–486
    [Google Scholar]
  13. Loomis W.F. 1975 Dictyostelium discoideum: A Developmental System New York: Academic Press;
    [Google Scholar]
  14. Loomis W.F. 1978a; Genetic analysis of the gene for N-acetylglucosaminidase in Dictyostelium discoideum. . Genetics 88:277–284
    [Google Scholar]
  15. Loomis W.F. 1978b; The number of developmental genes in Dictyostelium. . Birth Defects 14:497–505
    [Google Scholar]
  16. Loomis W.F. 1980; A β-glucosidase gene in Dictyostelium discoideum. . Developmental Genetics in the Press
    [Google Scholar]
  17. Loomis W.F., Dimond R.L., Free S.J., White S.S. 1977; Independent and dependent sequences in development of Dictyostelium. . In Eucaryotic Microbes as Model Developmental Systems pp. 177–194 Edited by O’Day D., Horgan P. New York: Marcel Dekker;
    [Google Scholar]
  18. Ma G., Firtel R.A. 1978; Regulation of the synthesis of two carbohydrate binding proteins in Dictyostelium discoideum. . Journal of Biological Chemistry 253:3924–3932
    [Google Scholar]
  19. Macleod C.L. 1979 Gene expression in Dictyostelium discoideum: a genetic and biochemical analysis Ph.D. thesis University of California, San Diego, California; U.S.A.:
    [Google Scholar]
  20. Macleod C., Firtel R., Papkoff J. 1980; Regulation of actin gene expression during spore germination in Dictyostelium discoideum. . Developmental Biology 76:263–274
    [Google Scholar]
  21. Metzenberg R. 1979; Implication of some genetic control mechanisms in Neurospora. . Microbiological Reviews 43:361–383
    [Google Scholar]
  22. Sadler J.R., Smith T.F. 1971; Mapping of the lactose operator. Journal of Molecular Biology 62:139–170
    [Google Scholar]
  23. Sussman M. 1965; Temporal, spatial, and quanti-tative control of enzyme activity during slime mold cytodifferentiation. Brookhaven Symposia in Biology 18:66–76
    [Google Scholar]
  24. Sussman M. 1966; Biochemical and genetic methods in the study of cellular slime mold development. Methods in Cell Physiology 2:397–410
    [Google Scholar]
  25. Watts D.J., Ashworth J.M. 1970; Growth of myxamoebae of the cellular slime mold D. discoideum in axenic culture. Biochemical Journal 119:171–174
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-120-1-273
Loading
/content/journal/micro/10.1099/00221287-120-1-273
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error