1887

Abstract

A non-pigmented strain of (Db10) was isolated from moribund flies. From this strain were isolated spontaneous mutants resistant to streptomycin (Db11) and nalidixic acid (Db12). Mutant Db11 was used for the isolation of two phages, J and K, which grew on Db10, Db11 and Db12, but not on three reference strains of Mutant Db11 was demonstrated to fulfil Koch’s postulates. Strain Db10 and its antibiotic-resistant derivatives were lethal to whether given in the food or by injection. Evidence for toxin(s) was found only in sterile supernatants from 7 d cultures. Such extracts contained proteolytic activity and inactivated the antibacterial activity in immune haemolymph from Phages J and K were used to isolate phage-resistant mutants of Db11. Three such mutants and their parental strain were investigated for their susceptibility to immune haemolymph from The parental strain was resistant to incubation with 90% haemolymph for 2h at 37 °C; all phage-resistant mutants were susceptible to the immune haemolymph with ‘killing times’ (i.e. the time required to kill 90% of the viable cells) ranging from 15 to 55 min. When the same strains were compared for their virulence to , the phage-resistant mutants had significantly reduced virulence. It is concluded that resistance to insect immunity plays an important role in the overall pathogenicity of

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1980-09-01
2021-05-06
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References

  1. Adams M. H. 1959 Bacteriophages. New York:: Interscience;
    [Google Scholar]
  2. Bertani G. 1951; Studies on lysogenesis. I. The mode of phage liberation by lysogenic Escherichia coli . Journal of Bacteriology 62:293–300
    [Google Scholar]
  3. Bertani G., Torheim B., Laurent T. 1967; Multiplication in Serratia of a bacteriophage originating from Escherichia coli: lysogenization and host-controlled variation. Virology 32:619–632
    [Google Scholar]
  4. Boman H. G., Monner D. A. 1975; Characterization of lipopolysaccharides from Escherichia coli K-12 mutants. Journal of Bacteriology 121:455–464
    [Google Scholar]
  5. Boman H. G., Nilsson I., Rasmuson B. 1972; Inducible antibacterial defence system in Drosophila . Nature; London: 237232–235
    [Google Scholar]
  6. Boman H. G., Nilsson-Faye I., Paul K., Rasmuson T. 1974; Insect immunity. I. Characteristics of an inducible cell-free antibacterial reaction in hemolymph of Sarnia cynthia pupae. Infection and Immunity 10:136–145
    [Google Scholar]
  7. Bucher G. E. 1959; Bacteria of grasshoppers of Western Canada. III. Frequency of occurrence, pathogenicity. Journal of Invertebrate Pathology 1:391–405
    [Google Scholar]
  8. Edlund T., Sidén I., Boman H. G. 1976; Evidence for two immune inhibitors from Bacillus thuringiensis interfering with the humoral defense system of saturniid pupae. Infection and Immunity 14:934–941
    [Google Scholar]
  9. Faye I., Pye A., Rasmuson T., Boman H. G., Boman I. A. 1975; Insect immunity. II. Simultaneous induction of antibacterial activity and selective synthesis of some hemolymph proteins in diapausing pupae of Hyalophora cecropia and Sarnia cynthia . Infection and Immunity 12:1426–1438
    [Google Scholar]
  10. Flyg C., Boman H. G. 1979; A strain of Serratia marcescens pathogenic for Drosophila melanogaster. . In Progress in Invertebrate Pathology Proceedings of the International Colloquium on Invertebrate Pathology and Xlth Annual Meeting of the Society for Invertebrate Pathology, Prague 1978 p. 70:
    [Google Scholar]
  11. Foulds J. D., Shemin D. 1969; Properties and characteristics of a bacteriocin from Serratia marcescens. . Journal of Bacteriology 99:655–660
    [Google Scholar]
  12. Grimont P.A.D., Grimont F. 1978; The genus Serratia . Annual Review of Microbiology 32:221–248
    [Google Scholar]
  13. Grimont P.A.D., Grimont F., Lysenko O. 1979; Species and biotype identification of Serratia strains associated with insects. Current Microbiology 2:139–142
    [Google Scholar]
  14. Hamon Y., Péron Y. 1961; Étude de la propriété bactériocinogène dans le genre Serratia. . Annales de l’Institut Pasteur 100:818–821
    [Google Scholar]
  15. Hultmark D., Steiner H., Rasmuson T., Boman H. G. 1980; Insect immunity. VI. Purification and properties of three inducible bactericidal proteins from hemolymph of immunized pupae of Hyalophora cecropia . European Journal of Biochemistry 106:7–16
    [Google Scholar]
  16. Kaska M. 1976; The toxicity of extracellular proteases of the bacterium Serratia marcescens for larvae of greater wax moth, Galleria mellonslla. . Journal of invertebrate Pathology 27:271
    [Google Scholar]
  17. Li E., Yousten A. A. 1975; Metalloprotease from Bacillus thuringiensis. . Applied Microbiology 30:354–361
    [Google Scholar]
  18. Lyerly D., Kreger A. 1979; Purification and characterization of a Serratia marcescens metalloprotease. Infection and Immunity 24:411–421
    [Google Scholar]
  19. Lysenko O. 1976; Chitinase of Serratia marcescens and its toxicity to insects. Journal of Invertebrate Pathology 27:385–386
    [Google Scholar]
  20. Matsumoto H., Tazaki T., Hosogaya S. 1973; A generalized transducing phage of Serratia marcescens. . Japanese Journal of Microbiology 17:473–479
    [Google Scholar]
  21. Poinar G. O., Wassink H.J.M., Leegwater-Van Der Linden M. E., Van Der Geest L.P.S. 1979; Serratia marcescens as a pathogen of tsetse flies. Acta tropica 11:7–9
    [Google Scholar]
  22. Prehm P., Stirm S., Jann B., Jann K., Boman H. G. 1976; Cell-wall lipopolysaccharides of ampicillin-resistant mutants of Escherichia coli K-12. European Journal of Biochemistry 66:369–77
    [Google Scholar]
  23. Reed L. J., Muench H. 1938; A simple method of estimating fifty per cent endpoints. American Journal of Hygiene 27:493–497
    [Google Scholar]
  24. Sidén I., Dalhammar G., Telander B., Boman H. G., Somerville H. 1979; Virulence factors in Bacillus thuringiensis: purification and properties of a protein inhibitor of immunity in insects. Journal of General Microbiology 114:45–52
    [Google Scholar]
  25. Winkler U., Heller K. B., Folle B. Pleiotropic consequences of mutations towards antibiotic-hypersensitivity in Serratia marcescens. . Archives of Microbiology 116:259–268
    [Google Scholar]
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