1887

Abstract

The assignment to linkage group VII of the coumarin sensitivity mutation , which leads to a loss of colony-forming ability on agar containing coumarin, was established using the linkage group VII markers and Complementation of the and mutations was an effective method of selecting heterozygous diploids at 211C, i.e. without requiring a temperature sensitivity mutation. A morphological mutation, , which affects the distribution of fruiting bodies was also assigned to linkage group VII. Both the and mutations were discovered in the strain NP187. Eight independently isolated, recessive mutations leading to resistance to 300 g CoCl ml were allelic with the mutation. The partially dominant mutation was shown to map in linkage group VII on the basis of its segregation relative to the and mutations and is almost certainly an allele of the locus. These results are consistent with there being only a single locus at which mutations can lead to resistance to high concentrations of CoCl. Two temperature sensitivity mutations and were also assigned to linkage group VII.

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1980-09-01
2021-07-31
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References

  1. Free S. J., Schimke R. T., Loomis W. F. 1976; The structural gene for α-mannosidase-1 in Dictyostelium discoideum . Genetics 84:159–174
    [Google Scholar]
  2. Loomis W. F. 1969; Temperature-sensitive mutants of Dictyostelium discoideum . Journal of Bacteriology 99:65–69
    [Google Scholar]
  3. Loomis W. F. 1975 Dictyostelium discoideum: A Developmental System. New York:: Academic Press.;
    [Google Scholar]
  4. Loomis W. F., Ashworth J. M. 1968; Plaque-size mutants of the cellular slime mouldDictyostelium discoideum . Journal of General Microbiology 53:181–186
    [Google Scholar]
  5. Mosses D., Williams K. L., Newell P. C. 1975; The use of mitotic crossing-over for genetic analysis in Dictyostelium discoideum: mapping of linkage group II. Journal of General Microbiology 90:247–259
    [Google Scholar]
  6. Newell P. C. 1978; Genetics of the cellular slime moulds. Annual Review of Genetics 12:69–93
    [Google Scholar]
  7. Newell P. C., Henderson R. F., Mosses D., Ratner D. I. 1977; Sensitivity to Bacillus subtilis: a novel system for selection of heterozygous diploids of Dictyostelium discoideum . Journal of General Microbiology 100:207–211
    [Google Scholar]
  8. North M. J., Williams K. L. 1978; Relationship between the axenic phenotype and sensitivity to ω-amino carboxylic acids in Dictyostelium discoideum . Journal of General Microbiology 107:223–230
    [Google Scholar]
  9. Ratner D. I., Newell P. C. 1978; Linkage analysis in Dictyostelium discoideum using multiply marked tester strains: establishment of linkage group VII and the reassessment of earlier linkage data. Journal of General Microbiology 109:225–236
    [Google Scholar]
  10. Robson G. E., Williams K. L. 1977; The mitotic chromosomes of the cellular slime mouldDictyostelium discoideum: a karyotype based on Giemsa banding. Journal of General Microbiology 99:191–200
    [Google Scholar]
  11. Ross F. M., Newell P. C. 1979; Genetics of aggregation pattern mutations in the cellular slime mouldDictyostelium discoideum . Journal of General Microbiology 115:289–300
    [Google Scholar]
  12. Rothman F. G., Alexander E. T. 1975; Parasexual genetic analysis of the cellular slime mold Dictyostelium discoideum A3. Genetics 80:715–731
    [Google Scholar]
  13. Sussman M. 1966; Biochemical and genetic methods in the study of cellular slime mold development. Methods in Cell Physiology 2:397–410
    [Google Scholar]
  14. Welker D. L., Deering R. A. 1976; Genetic analysis of radiation-sensitive mutations in the slime mouldDictyostelium discoideum . Journal of General Microbiology 97:1–10
    [Google Scholar]
  15. Welker D. L., Deering R. A. 1978; Genetics of radiation sensitivity in the slime mould Dicryostelium discoideurn . Jourtral of General Microbiology 109:11–23
    [Google Scholar]
  16. Williams K. L. 1978; Characterization of dominant resistance to cobalt chloride in Dictyostelium discoideum and its use in parasexual genetic analysis. Genetics 90:37–47
    [Google Scholar]
  17. Williams K. L., Barrand P. 1978; Parasexual genetics in the cellular slime mouldDictyostelium discoideum:haploidisation of diploid strains using ben late. FEMS Microbiology Letters 4:155–159
    [Google Scholar]
  18. Williams K. L., Newell P. C. 1976; A genetic study of aggregation in the cellular slime mouldDictyostelium discoideum using complementation analysts. Genetics 82:287–307
    [Google Scholar]
  19. Williams K. L., Welker D. L. 1980 Developmental Genetics (in the Press)
    [Google Scholar]
  20. Williams K. L., Kessin R. H., Newell P. C. 1974; Parasexual genetics in Dictyostelium discoideum: mitotic analysis of acriflavin resistance and growth in axenic medium. Journal of General Microbiology 84:59–69
    [Google Scholar]
  21. Wright M. D., Williams K. L., Newell P. C. 1977; Ethidium bromide resistance, a selective marker located on linkage group IV of Dictyostelium discoideum . Journal of General Microbiology 102:423–426
    [Google Scholar]
  22. Zada-Hames I. M. 1977; Analysis of karyotype and ploidy of Dictyostelium discoideum using colchicine-induced metaphase arrest. Journal of General Microbiology 99:201–208
    [Google Scholar]
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