1887

Abstract

The aminoadipic acid pathway has previously been found to function as a biosynthetic route to lysine in higher fungi and as a catabolic pathway in mammals and higher plants. Results are now presented which suggest that in (fungi imperfecti), the aminoadipic acid pathway is important in both the biosynthesis and catabolism of lysine. experiments using L-[4-C]aspartate showed that 98% of the radioactivity in protein-bound lysine and aspartate was in the latter, and with -amino[1-C]adipic acid radioactivity was readily incorporated into saccharopine and free and protein-bound lysine. Endogenously supplied aminoadipic acid and saccharopine caused a rapid and substantial rise in the concentrations of free lysine in the mycelium. Cell-free extracts catalysed the conversion of aminoadipic acid to saccharopine and of saccharopine to lysine. and several other fungi were found to contain significant quantities of the intermediate saccharopine. The concentration of saccharopine increased when was supplied with lysine, and mycelia converted L-[C]lysine into saccharopine and 2-amino-adipic acid. In addition, all of the enzyme activities necessary to break lysine down to 2-oxoadipic acid were demonstrated in extracts.

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1980-09-01
2024-04-19
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References

  1. Broquist H. P., Trupin J. S. 1966; Amino acid metabolism. Annual Review of Biochemistry 35:231–274
    [Google Scholar]
  2. Davies H. M., Miflin B. J. 1978; Advantage of o-phthalaldehyde for visualizing 14C-labelled amino acids on thin layer chromatograms and an improved method for their recovery. Journal Of Chromatography 153:284–286
    [Google Scholar]
  3. Fellows F.C.I. 1973; Biosynthesis and degradation of saccharopine, an intermediate of lysine metabolism. Biochemical Journal 136:321–327
    [Google Scholar]
  4. Fellows F.C.1., Lewis M. H. R. 1973; Lysine metabolism in mammals. Biochemical Journal 136:329–334
    [Google Scholar]
  5. Fjellstadt T. A., Robinson J. C. 1975; Properties of partially purified saccharopine dehydrogenase from human placenta. Archives of Biochemistry and Biophysics 171:191–196
    [Google Scholar]
  6. Guengerich F. P., Broquist H. P. 1976; Lysine catabolism in Rhizoctonialeguminicola and related fungi. Journal of Bacteriology 126:338–347
    [Google Scholar]
  7. Higashino K., Fujioka M., Yamamura Y. 1971; The conversion of l-lysine to saccharopineand α-aminoadipate in mouse. Archives of Biochemistry and Biophysics 142:606–614
    [Google Scholar]
  8. Hutzler J., Dancis J. 1968; Conversion of lysine to saccharopine by human tissues. Biochimica et biophysica acta 158:62–69
    [Google Scholar]
  9. Hutzler J., Odievre M., Dancis J. 1968; Analysis for lysine, arginine, histidine and tyrosine in biological fluids. Analytical Biochemistry 19:529–541
    [Google Scholar]
  10. Kirkman M. A. 1974; Comparative determination of protein amino acids in plant materials by automated cation exchange and gas liquid chromatography of the amino acids N-heptafluoro-butyryl n-propyl esters. Journal of Chromatography 97:175–191
    [Google Scholar]
  11. Kuo M. H., Saunders P. P., Broquist H. P. 1964; Lysine biosynthesis in yeast: a new metabolite of α-aminoadipic acid. Journal of Biological Chemistry 239:508–515
    [Google Scholar]
  12. Kurtz M., Bhattacharjee J. K. 1975; Biosynthesis of lysine in Rhodotorula glutinis: role of pipecolic acid. Journal of General Microbiology 86:103–110
    [Google Scholar]
  13. Larson R. L., Sandine W. D., Broquist H. P. 1963; Enzymic reduction of α-aminoadipic acid; relation to lysine biosynthesis. Journal of Biological Chemistry 238:275–282
    [Google Scholar]
  14. Meister A. 1965 Biochemistry of the Amino Acids, 2nd edn.. New York: Academic Press.;
    [Google Scholar]
  15. Moller B. L. 1974; Lysine biosynthesis in barley (Hordeumvulgare L.). Plant Physiology 54:638–643
    [Google Scholar]
  16. Moller B. L. 1976a; Lysine catabolism in barley (Hordeumvulgare L.). Plant Physiology 57:687–692
    [Google Scholar]
  17. Moller B. L. 1976b; Conversion of saccharopine to lysine in barley. Phytochemistry 15:695–696
    [Google Scholar]
  18. Rothstein M., Miller L. L. 1954; The metabolism of l-lysine-6-C14. Journal of Biological Chemistry 211:851–858
    [Google Scholar]
  19. Schweet R. S., Holden J. T., Lowery P. H. 1955 In William D. McElroy atui H. Bentley Class, Johns Hopkins University Symposium, Amino Acid Metabolism p. 496 Baltimore: The Johns Hopkins Press.;
    [Google Scholar]
  20. Soda K., Misono H. 1968; l-Lysine-α-keto-glutarateaminotransferase II. Purification, crystallisation and properties. Biochemistry 7:4110–4119
    [Google Scholar]
  21. Soda K., Misono H., Yamamoto T. 1968; l-Lysine-α-ketoglutarate aminotransferase I. Identification of a product, Δ1-piperideine-6-carboxylic acid. Biochemistry 7:4102–4109
    [Google Scholar]
  22. Trupin J. S., Broquist H. P. 1965; Saccharopine, an intermediate of the aminoadipic acid pathway in lysine biosynthesis. Journal of Biological Chemistry 240:2524–2530
    [Google Scholar]
  23. Vogel J. H. 1964; Distribution of lysine pathways among fungi: evolutionary implications. American Naturalist 98:435–446
    [Google Scholar]
  24. Vogel H. J., Thompson J. S., Shockman G. D. 1970; Characteristic metabolic patterns of prokaryotes and eukaryotes. Symposia of the Society for General Microbiology 20:107–119
    [Google Scholar]
  25. Wade M. 1977 Investigations into the design of a selective fungicide based on differential pathways of lysine biosynthesis. Ph.D. thesis: London University;
    [Google Scholar]
  26. Wallsgrove R. M., Lea P. J., Mills W. R., Miflin B. J. 1979; The regulation and subcellular distribution of enzymes of the aspartate pathway in Pisumsativum leaves. Plant Physiology 63: Suppl. 26
    [Google Scholar]
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