1887

Abstract

SUMMARY: strain Duncan readily synthesized the adaptive (inducible) enzyme -galactosidase in washed suspension when supplied with a mixture of amino acids and an inducer. The amount of enzyme produced was a function of the quantity of amino acids supplied and the rate of synthesis was determined by the concentration of inducer in the system. Galactose was a highly active inducer, lactose a relatively feeble one. Production of the enzyme ensued after a lag period which could be shortened by the addition of glucose or sodium lactate and further shortened by supplying a mixture of purines and pyrimidines to the system. Enzyme formation was strongly inhibited by various antibacterial agents.

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/content/journal/micro/10.1099/00221287-12-2-288
1955-04-01
2021-10-19
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