1887

Abstract

SUMMARY:The staphylococcal typing phages require divalent cations for a stage in phage growth which is probably that of penetration. When phage is adsorbed to the cell surface of sensitive cocci and a chelating agent is added, there is a short period during which the phage particle may be inactivated and its lethal effect on the coccus prevented. Cocci attacked by phages of serological group A show penetration of the phages in distilled water + CaCl, or + MgCl, or to a less extent + SrCl. The phages of group B are unable to penetrate in presence of SrCl and in some cases MgCl is only partially active as compared with CaCl. Ca ions appear to be required specifically at a late stage in phage synthesis since the average yield of phage/coccus increases with increasing concentration of CaCl; the addition of a chelating agent towards the end of the latent period depresses the yield of phage.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-12-2-275
1955-04-01
2022-09-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/12/2/mic-12-2-275.html?itemId=/content/journal/micro/10.1099/00221287-12-2-275&mimeType=html&fmt=ahah

References

  1. Adams M.H. 1949; The calcium requirement of coli phage T5. . J. Immunol. 62:505
    [Google Scholar]
  2. Beumer J., Beumer-Jochmans M.P. 1951; Les exigences en calcium du phage de Lisbonne. Ann. Inst.Pasteur 81:489
    [Google Scholar]
  3. Delbrück. 1948; Biochemical mutants of bacterial viruses. J. Bact. 56:1
    [Google Scholar]
  4. Fildes P. 1953; Factors affecting the multiplication of bacteriophage. VI Congr. int. Microbiol. Symp. Mutation and Growth Factors p. 184
    [Google Scholar]
  5. Fildes P., Kay D., Joklik W.K. 1953; Divalent metals in phage production. Symp. Soc. gen. Microbiol. 2:194
    [Google Scholar]
  6. Luria S.E., Steiner D.L. 1954; The role of calcium in the penetration of bacteriophage T5 into its host. J. Bact. 67:635
    [Google Scholar]
  7. Potter N.N., Nelson F.E. 1953; Role of calcium and related ions in proliferation of lactic streptococcus bacteriophage. J. Bact. 66:508
    [Google Scholar]
  8. Puck T.T. 1953; The first steps of virus invasion. Cold Spr. Harb. Symp. quant. Biol. 18:149
    [Google Scholar]
  9. Rountree P.M. 1949; The serological differentiation of staphylococcal bacteriophages. J. gen. Microbiol. 3:164
    [Google Scholar]
  10. Rountree P.M. 1951; The role of certain electrolytes in the adsorption of staphylococcal bacteriophages. J. gen. Microbiol. 5:673
    [Google Scholar]
  11. WilliamsSmith H. 1948; Calcium-deficient media: their effect on phage action. Nature; Lond.: 161397
    [Google Scholar]
  12. Wilson G.S., Atkinson J.D. 1945; Typing of staphylococci by the bacteriophage method. Lancet i:647
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-12-2-275
Loading
/content/journal/micro/10.1099/00221287-12-2-275
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error