1887

Abstract

SUMMARY: Phage S13, a small phage active against a strain of , had a latent period of 20 min. on this host. Intracellular phage multiplication was detected only toward the end of the latent period. Photo-reactivation of ultraviolet irradiated phage occurred, but the inactivated phage did not kill its host. More than 1600 compounds were tested for ability to prevent growth of phage S13 on at concentrations allowing growth of the bacterium, but only thirteen were active. Seven of these compounds were examined further; none affected free S13 phage or the adsorption of S13 phage to its host. The only compound investigated in detail, 4:4-bis-(2-dihydroglyoxalinyl) stilbene dihydrochloride, inhibited both early and late, but probably not intermediate, stages in the intracellular development of S13 phage. This was different from the action of this compound on T1 phage. No relationship was found between structure and action against phage, nor between compounds active against S13 phage and those active against larger phages.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-12-2-172
1955-04-01
2021-10-16
Loading full text...

Full text loading...

/deliver/fulltext/micro/12/2/mic-12-2-172.html?itemId=/content/journal/micro/10.1099/00221287-12-2-172&mimeType=html&fmt=ahah

References

  1. Bertant B., Weigle J.J. 1953; Host controlled variation in bacterial viruses. J. Bact. 65:113
    [Google Scholar]
  2. Chantrill B.H., Coulthard C.E., Dickinson L., Inkley G.W., Morris W., Pyle A.H.J. 1952; The action of plant extracts on a bacteriophage of Pseudomonas pyocyanea and on influenza A virus. J. gen. Microbiol. 6:74
    [Google Scholar]
  3. Dickinson L. 1948; The bacteriophages of Pseudomonas pyocyanea. (1) The effect of various substances on their development. J. gen. Microbiol. 2:154
    [Google Scholar]
  4. Dickinson L., Chantrill B.H., Inkley G.W., Thompson M.J. 1953; The antiviral action of phenanthridinium compounds. Brit. J. Pharmacol. 8:139
    [Google Scholar]
  5. Dickinson L., Codd S. 1952; The bacteriophages of Pseudomonas pyocyanea. (2) Bacteriophage reproduction in an iridescent strain. J. gen. Microbiol. 6:1
    [Google Scholar]
  6. Dulbecco R. 1952; A critical test of the recombination theory of multiplicity reactivation. J. Bact. 63:199
    [Google Scholar]
  7. Elford W.J. 1936; Centrifugation studies: (1) Critical examination of a new method as applied to the sedimentation of bacteria, bacteriophages and proteins. Brit. J. exp. Path. 17:399
    [Google Scholar]
  8. Elford W.J., Andrewes C.H. 1932; Sizes of different bacteriophages. Brit. J. exp. Path. 13:446
    [Google Scholar]
  9. Foster R.A.C. 1948; An analysis of the action of proflavine on bacteriophage growth. J. Bact. 56:795
    [Google Scholar]
  10. Hotchin J.E. 1951; Influence of acridines on interaction of Staphylococcus aureus and staphylococcus phage K. J. gen. Microbiol. 5:609
    [Google Scholar]
  11. Mills R.F.N. 1953; The action of 2 : 7-bis-(2´-dihydroglyoxalinyl)-9-phenyl-phenanthridine on a bacteriophage of Pseudomonas pyocyanea . Brit. J. Pharmacol. 8:143
    [Google Scholar]
  12. Rountree P.M. 1951; A complement-fixing antigen of Bact. colibacteriophage T5: its behaviour during virus growth. Brit. J. exp. Path. 32:341
    [Google Scholar]
  13. Swanstrom M., Adams M.H. 1951; Agar layer method for production of high titre phage stocks. Proc. Soc. exp. Biol., N.Y. 78:372
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-12-2-172
Loading
/content/journal/micro/10.1099/00221287-12-2-172
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error