The Metabolism of Sodium 2-Keto--gluconate by Micro -organisms Free

Abstract

SUMMARY: The ability of various bacteria, actinomycetes, yeasts and moulds to grow on a medium containing sodium 2-keto--gluconate as the major carbon source was investigated. The disappearance of 2-ketogluconate during growth was followed and the ability of washed unadapted cell suspensions to oxidize this substrate (as evidenced by O uptake) was studied. Adapted strains were examined for the presence of a 2-ketogluconokinase; this enzyme was detected in organisms of the genera Although some bacteria and yeasts consumed 2-ketogluconate during growth and washed cells were able to oxidize it (after an induction period) 2-ketogluconokinase activity was not detected in cell-free extracts, prepared from these organisms, namely: species of Several moulds (chiefly Pyrenomycetes, Aspergillales and Fungi Imperfecti) displayed the same phenomena. A few strains grew weakly on the substrate; however, unadapted cells did not show uptake of oxygen. The remaining strains of bacteria, yeasts, moulds and all the actinomycetes were without activity on 2-ketogluconate.

The results in this paper substantiate the opinion that the ‘direct oxidation’ pathways of carbohydrate metabolism are very widespread and important among microorganisms.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-12-2-162
1955-04-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/12/2/mic-12-2-162.html?itemId=/content/journal/micro/10.1099/00221287-12-2-162&mimeType=html&fmt=ahah

References

  1. Bergey’s Manual of Determinative Bacteriology 1948, 6th ed.. Breed R.S., Murray E.G.D., Hitchens A.P. Ed. Baltimore: The Williams & Wilkins Co;
    [Google Scholar]
  2. De donder R. 1952; Quelques aspects de la glycolyse chez Bac. subtilis et Bac. megatherium. Commun. Ini. Cong. Biochem. pp. 77
    [Google Scholar]
  3. De ley J. 1953a; Over de directe Oxydatie van Suikers en Suikerderivaten door Micro-organismen. Meded. vlaamsche Acad, geneesk. Belg. pp. 34
    [Google Scholar]
  4. De ley J. 1953b; The oxidation of some carbohydrates by Aerobacter cloacae and their connexion with the direct oxidation scheme. Enzymologia 16:14
    [Google Scholar]
  5. De ley J. 1953c; The phosphorylation of some carbohydrates, connected with the direct oxidation by Aerobacter cloacae. Enzymologia 16:99
    [Google Scholar]
  6. De ley J. 1954a; Phospho-2-keto-d-gluconate, an intermediate in the carbohydrate metabolism of Aerobacter cloacae. Biochim. biophys.Acta 13:302
    [Google Scholar]
  7. De ley J. 1954b; 2-Keto-d-gluconate-6-phosphate, a new intermediate in the carbohydrate metabolism of Aerobacter cloacae. Enzymologia 17:55
    [Google Scholar]
  8. Entner N., Doudoroff M. 1952; Glucose and gluconic acid oxidation of Pseudomonas saccharophila. J. biol. Chem. 196:853
    [Google Scholar]
  9. Entner N., Stanier R.Y. 1951; Studies on the oxidation of glucose by Pseudomonas fluorescens. J. Bact. 62:181
    [Google Scholar]
  10. Gomori G. 1946; Buffers in the range of pH 6·5 to 9·6. Proc. Soc. exp. Biol., N.Y. 62:83
    [Google Scholar]
  11. Hughes D.E. 1951; A press for disrupting bacteria and other micro-organisms. Brit.J. exp. Path. 32:97
    [Google Scholar]
  12. Klein H.P. 1953; Some properties of the hexokinase of Pseudomonas putrefadens. J. Bact. 66:650
    [Google Scholar]
  13. Koffler H. 1953; Problems of oxidative mold metabolism. Vlth Int. Cong. Microbiol. Symposium on Microbial Metabolism pp. 38
    [Google Scholar]
  14. Lodder J., Kreger-van Rij N.J.W. 1952 The Yeasts, a Taxonomic Study. Amsterdam:: North-Holland Publishing Co.;
    [Google Scholar]
  15. McIlwain H. 1948; Preparation of cell-free bacterial extracts with powdered alumina. J. gen. Microbiol. 2:288
    [Google Scholar]
  16. Mcnair Scott D.B., Cohen S.S. 1951; The origin and metabolism of ribose. J. cell. comp. Physiol. 38:178 Suppl. 1.
    [Google Scholar]
  17. Mickle H. 1948; Tissue disintegrator. J. R. micr. Soc. 68:10
    [Google Scholar]
  18. Ohle H., Wolter R. 1930; Zur Kenntnis der 2-Keto-glukonsaure (II. Mitteil.). Ber. dtsch. chem. Ges. 63:843
    [Google Scholar]
  19. Report of the Coliform Sub-committee 1949; The classification of the coli-aerogenes bacteria. Proc. Soc. appl. Bact. pp. 3
    [Google Scholar]
  20. Schoorl N. 1912; Het reducerend Vermogen van Suikers. Chem. Weekbl. 9:678
    [Google Scholar]
  21. Stokes F.N., Campbell J.J.R. 1951; The oxidation of glucose and gluconic acid by dried cells of Pseudomonas aeruginosa. Arch. Biochem. 30:121
    [Google Scholar]
  22. Wood W.A., Schwert R.F. 1953; Carbohydrate oxidation by Pseudomonas fluorescens. I. The mechanism of glucose and gluconate oxidation. J. biol. Chem. 201:501
    [Google Scholar]
  23. Wood W.A., Schwert R.F. 1954; Carbohydrate oxidation by Pseudomonas fluorescens. II. Mechanism of hexose phosphate oxidation. J. biol. Chem. 20:6625
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-12-2-162
Loading
/content/journal/micro/10.1099/00221287-12-2-162
Loading

Data & Media loading...

Most cited Most Cited RSS feed