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A regulatory mutation has been identified that reduces expression of asparaginase II in exponential phase cells of Saccharomyces cerevisiae to 25 % or less of the wild-type activity. Mutants in this gene (ASP4) can be derepressed for asparaginase II by nitrogen starvation and will grow with D-asparagine as the sole nitrogen source. ASP4 assorts independently from ASP2 and ASP3. Mutation in either of the latter two genes results in lack of asparaginase II activity under any growth conditions.
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