1887

Abstract

The cervical microbial flora of 25 females and stock cultures of various micro-organisms which may be present in the human female cervix were examined using a fluorimetric assay for 1,2-propanediol oxidoreductase. Results indicated that only members of the genera and possess appreciable activities of the enzyme, whose physiological function is not yet known. The activity of this enzyme in appeared to be significantly higher than the activities observed in most of the other species and in the species. These results indicated that it may be possible to utilize this enzyme as a presumptive diagnostic marker for in cervical secretions. 1,2-Propanediol oxidoreductase may also be of taxonomic significance for the classification of various bacterial species.

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/content/journal/micro/10.1099/00221287-119-2-459
1980-08-01
2024-04-23
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References

  1. Blackwell C., Young H., Bain S. S. R. 1977; Isolation of Neisseria meningitidis and Neisseria catarrhalis from the genitourinary tract and anal canal.. British Journal of Venereal Diseases 54:41–44
    [Google Scholar]
  2. Burton R. M. 1955; Glycerol dehydrogenase from Aerobacter aerogenes. . Methods in Enzymology 1:397–400
    [Google Scholar]
  3. Catlin B. W. 1970; Transfer of the organism named Neisseria catarrhalis to Branhamella gen.nov.. International Journal of Systematic Bacteriology 20:155–159
    [Google Scholar]
  4. Corbishley C. M. 1977; Microbial flora of the vagina and cervix.. Journal of Clinical Pathology 30:745–748
    [Google Scholar]
  5. Faur Y. C., Weisburd M. H., Wilson M. E. 1975; Isolation of Neisseria meningitidis from the genito-urinary tract and anal canal.. Journal of Clinical Microbiology 2:178–182
    [Google Scholar]
  6. Givan K. F., Thomas B. W., Johnston A. G. 1977; Isolation of Neisseria meningitidis from urethra, cervix, and anal canal: further observations.. British Journal of Venereal Diseases 53:109–112
    [Google Scholar]
  7. Kellogg D. S. Jr Hill G. A. 1976; Laboratory diagnosis of gonorrhea.. In Cumitech 4 pp. 6–8 Marcus S., Sherris J. C. Edited by Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  8. Lewis J. F., Alexander J. J. 1974; Isolation of Neisseria meningitidis from the vagina and cervix.. American Journal of Clinical Pathology 61:216–217
    [Google Scholar]
  9. Lowe D. A., Turner J. M. 1968; Enzymic oxidation of d-1-amino-2-propanol BY diol dehydrogenases of microbial origin.. Biochimica et biophysica acta 170:455–456
    [Google Scholar]
  10. Lowe D. A., Turner J. M. 1970; Microbial metabolism of amino ketones: d-l-aminopropan-2-ol and aminoacetone metabolism in Escherichia coli. . Journal of General Microbiology 63:49–61
    [Google Scholar]
  11. Mcdonald H. C., Takeguchi M. M., Detar C. C., Simon P. A., Livsey K. A., Odstrchel G., Kaplan N. O., Weetall H. H. 1980; The isolation and characterization of a 1,2-propanediol oxidoreductase from Neisseria gonorrhoeae. . Journal of General Microbiology 119:451–458
    [Google Scholar]
  12. Sridhara S., Wu T. T., Chused T. M., Lin E. C. C. 1969; Ferrous-activated nicotinamide adenine dinucleotide-linked dehydrogenase from a mutant of Escherichia coli capable of growth on 1,2-propanediol.. Journal of Bacteriology 98:87–95
    [Google Scholar]
  13. Tanaka Y., Fujii K., Tanaka A., Fukui S. 1975; Properties of 1,2-propanediol-dehydrogenating enzyme from a 1,2-propanediol-utilizing soil bacterium.. Journal of Fermentation Technology 53:566–568
    [Google Scholar]
  14. Tatum H. W., Ewing W. H., Weaver R. E. 1974; Miscellaneous Gram-negative bacteria.. In Manual of Clinical Microbiology, 2nd edn.. pp. 274–276 Lennette E. H., Spaulding E. H., Truant J. P. Edited by Washington, D.C.: American Society for Microbiology;
    [Google Scholar]
  15. Worthington Enzymem Manual 1972 p. 5 Freehold, New Jersey: Worthington Biochemical Corporation;
  16. Wu T. T. 1976; Growth on d-arabitol of a mutant strain of Escherichia coli K12 using a novel dehydrogenase and enzymes related to L-1,2-propanediol and d-xylose metabolism.. Journal of General Microbiology 94:246–256
    [Google Scholar]
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