1887

Abstract

An enzyme which oxidizes 1,2-propanediol in the presence of NADhas been purified from lysates of The enzyme was activated by monovalent cations, had a pH optimum between 9 and 10, and showed a substrate specificity unlike any known alcohol or glycerol dehydrogenase. The enzyme had an apparent of 17 m for 1,2-propanediol and 0.37 mM for NAD. When chromatographed on a Sephadex G-150 column, the enzyme eluted as a single peak in the molecular weight region of a bovine serum albumin marker. An antibody to the purified enzyme was prepared in goats. When antiserum was reacted with the enzyme in immunodiffusion experiments, a single precipitin band was detected. When the enzyme was mixed with an excess of antibody and then reacted with substrate, enzyme activity was completely inhibited.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-119-2-451
1980-08-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/119/2/mic-119-2-451.html?itemId=/content/journal/micro/10.1099/00221287-119-2-451&mimeType=html&fmt=ahah

References

  1. Axen R., Porath J., Ernback S. 1967; Chemical coupling of peptides and proteins to polysaccharide by means of cyanogen halides.. Nature; London: 2141302–1304
    [Google Scholar]
  2. Boronat A., Aguilar J. 1979; Rhamnose-induced propanediol oxidoreductase in Escherichia coli: purification, properties, and comparison with the fucose-induced enzyme.. Journal of Bacteriology 140:320–326
    [Google Scholar]
  3. Cocks G. T., Aguilar J., Lin E. C. C. 1974; Evolution of 1,2-propanediol catabolism in Escherichia coli by recruitment of enzymes for l-fucose and l-lactate metabolism.. Journal of Bacteriology 118:83–88
    [Google Scholar]
  4. Corbishley C. M. 1977; Microbial flora of the vagina and cervix.. Journal of Clinical Pathology 30:745–748
    [Google Scholar]
  5. Lee C. Y., Kaplan N. O. 1975; Characteristics of 8-substituted adenine nucleotide derivatives utilized in affinity chromatography.. Archives of Biochemistry and Biophysics 168:665–676
    [Google Scholar]
  6. Lee C. Y., Lappi D. A., Wermuth B., Everse J., Kaplan N. O. 1974; 8-(6-Aminohexyl)-aminoadenine nucleotide derivatives for affinity chromatography.. Archives of Biochemistry and Biophysics 163:561–569
    [Google Scholar]
  7. Lowe D. A., Turner J. M. 1968; Enzymic oxidation of D-l-amino-2-propanol by diol dehydrogenases of microbial origin.. Biochimica et biophysica acta 170:455–456
    [Google Scholar]
  8. Lowe D. A., Turner J. M. 1970; Microbial metabolism of amino ketones: d-1-aminopropan- 2-ol and aminoacetone metabolism in Escherichia coli.. Journal of General Microbiology 63:49–61
    [Google Scholar]
  9. Maurer P. H. 1971; The quantitative precipitin reaction.. Methods in Immunology and Immuno-chemistry 3:1–58
    [Google Scholar]
  10. Mcdonald H. C., Weetall H. H., Simon P., Takeguchi M., Odstrchel G. 1978; Characterization of an immobilized antibody-enzyme complex.. Journal of Solid-Phase Biochemistry 3:151–160
    [Google Scholar]
  11. Owen P., Smyth C. J. 1977; Enzyme analysis by quantitative immunoelectrophoresis.. In Immuno-chemistry of Enzymes and Their Antibodies pp. 147–202 Salton M. R. J. Edited by New York:: John Wiley.;
    [Google Scholar]
  12. Smyth C. J., Friedman-Klein A. E., Salton M. R. J. 1976; Antigenic analysis of Neisseria gonorrhoeae by crossed immunoelectrophoresis.. Infection and Immunity 13:1273–1288
    [Google Scholar]
  13. Sridhara S., Wu T. T., Chused T. M., Lin E. C. C. 1969; Ferrous-activated nicotinamide adenine dinucleotide-linked dehydrogenase from a mutant of Escherichia coli capable of growth on 1,2-propanediol.. Journal of Bacteriology 98:87–95
    [Google Scholar]
  14. Takeguchi M. M., Livsey K. A., Detar C. C., Mcdonald H. C., Smith D. K., Simon P. A., Weetall H. H. 1980; The occurrence of 1,2-propanediol oxidoreductase in micro-organisms and its use as a possible diagnostic marker for Neisseria gonorrhoeae.. Journal of General Microbiology 119:459–464
    [Google Scholar]
  15. Tanaka J., Fujii K., Tanaka A., Fukui S. 1975; Properties of 1,2-propanediol-dehydrogen-ating enzyme from a 1,2-propanediol-utilizing soil bacterium.. Journal of Fermentation Technology 53:566–568
    [Google Scholar]
  16. Tauber H., Russell L. 1962; Enzymes of Neisseria gonorrhoeae and other Neisseria.. Proceedings of the Society for Experimental Biology and Medicine 110:440–443
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-119-2-451
Loading
/content/journal/micro/10.1099/00221287-119-2-451
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error