1887

Abstract

Non-lysogenic strain 1030 was lysogenized with 12 different bacteriophages. Lysogeny was associated with acquisition of phage inducibility by mitomycin C treatment or ultraviolet irradiation, with the presence of plaque-forming phage in culture supernatants and with considerable narrowing in susceptibility to the typing bacteriophages and also with increased sensitivity to trimethoprim and sulphadiazine. The presence of prophages in the donor and/or the recipient could either promote or inhibit transfer of plasmids between mixed cultures. Transfer frequencies in mixed culture after 18 h incubation could be as high as 7.0 × 10resistant recipients/final donor, and evidence was adduced for a mechanism distinct from transfer by spontaneous transduction. It is suggested that this method of gene transfer be described as “phage-mediated conjugation”. Chromosomal genes were not transferred by this method. Two similar plasmids -2 and -3 were able to promote their own transfer through clones of 1030; plasmids coding for resistance to either neomycin or tetracycline could be transferred to a recipient by the presence of -2 or -3 simultaneously in the donor. The presence of plasmids in 1030 was associated with a small increase in sensitivity to trimethoprim or sulphadiazine.

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/content/journal/micro/10.1099/00221287-119-2-423
1980-08-01
2021-05-07
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References

  1. Chopra I., Bennett P., Lacey R. W. 1973; A variety of staphylococcal plasmids present as multiple copies.. Journal of General Microbiology 79:343–345
    [Google Scholar]
  2. Devoret R., Blanco M. 1970; Mutants of variety of staphylococcal plasmids Escherichia coli K12 (λ)+ non-inducible by thy mine starvation.. Molecular and General Genetics 107:272–280
    [Google Scholar]
  3. Lacey R. W. 1971a; High-frequency transfer of neomycin resistance between naturally-occurring strains of Staphylococcus aureus.. Journal of Medical Microbiology 4:73–84
    [Google Scholar]
  4. Lacey R. W. 1971b; Transfer of tetracycline resistance between strains of Staphylococcus aureus in mixed cultures.. Journal of General Microbiology 69:229–237
    [Google Scholar]
  5. Lacey R. W. 1972; Genetic control in methicillin- resistant strains of Staphylococcus aureus.. Journal of Medical Microbiology 5:497–508
    [Google Scholar]
  6. Lacey R. W. 1975; Antibiotic resistance plasmids of Staphylococcus aureus and their clinical importance. . Bacteriological Reviews 39:1–32
    [Google Scholar]
  7. Lacey R. W. 1979; An unusual element from Staphylococcus aureus.. Journal of Medical Microbiology 12:311–320
    [Google Scholar]
  8. Lacey R. W., Grinsted J. 1973; Genetic analysis of methicillin-resistant strains of Staphylococcus aureus: evidence for their evolution from a single clone.. Journal of Medical Microbiology 6:511–526
    [Google Scholar]
  9. Lacey R. W., Stokes A. 1979; Studies on recently isolated cultures of methicillin-resistant Staphylococcus aureus.. Journal of General Microbiology 114:329–339
    [Google Scholar]
  10. Lantos J. 1977; Transfer of erythromycin resistance in Staphylococcus aureus. . Acta microbiologica Academiae scientiarum hungaricae 24:107–113
    [Google Scholar]
  11. Lindberg M., Novick R. P. 1973; Plasmid- specific transformation in Staphylococcus aureus. . Journal of Bacteriology 115:139–145
    [Google Scholar]
  12. Melechen N. E. 1962; The provocation of an early step of induction by thymine deprivation.. Virology 16:21–29
    [Google Scholar]
  13. Naidoo J., Noble W. C. 1978; Transfer of gentamicin resistance between strains of Staphylococcus aureus on skin. . Journal of General Microbiology 107:391–393
    [Google Scholar]
  14. Novick R. P. 1963; Analysis by transduction of mutations affecting penicillinase formation in Staphylococcus aureus. . Journal of General Microbiology 33:121–136
    [Google Scholar]
  15. Novick R. P. 1967; Properties of a cryptic high- frequency transducing phage in Staphylococcus aureus. . Virology 33:155–166
    [Google Scholar]
  16. Novick R. P., Morse S. I. 1967; In vivo transmission of drug resistance factors between strains of Staphylococcus aureus. . Journal of Experimental Medicine 125:45–59
    [Google Scholar]
  17. Pattee P. A., Baldwin J. N. 1961; Transduction of resistance to some macrolide antibiotics in Staphylococcus aureus.. Journal of Bacteriology 84:875–881
    [Google Scholar]
  18. Richmond M. H. 1969; Extrachromosomal elements and the spread of antibiotic resistance in bacteria.. Biochemical Journal 113:225–235
    [Google Scholar]
  19. Robinson M. K., Bennett P. M., Richmond M. H. 1977; Inhibition of TnA translocation by TnA.. Journal of Bacteriology 129:407–414
    [Google Scholar]
  20. Witte W. 1977; Transfer of drug resistance plasmids in mixed cultures of staphylococci. . Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene 237:147–159
    [Google Scholar]
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