Summary: Non-lysogenic strain 1030 was lysogenized with 12 different bacteriophages. Lysogeny was associated with acquisition of phage inducibility by mitomycin C treatment or ultraviolet irradiation, with the presence of plaque-forming phage in culture supernatants and with considerable narrowing in susceptibility to the typing bacteriophages and also with increased sensitivity to trimethoprim and sulphadiazine. The presence of prophages in the donor and/or the recipient could either promote or inhibit transfer of plasmids between mixed cultures. Transfer frequencies in mixed culture after 18 h incubation could be as high as 7.0 × 10resistant recipients/final donor, and evidence was adduced for a mechanism distinct from transfer by spontaneous transduction. It is suggested that this method of gene transfer be described as “phage-mediated conjugation”. Chromosomal genes were not transferred by this method. Two similar plasmids -2 and -3 were able to promote their own transfer through clones of 1030; plasmids coding for resistance to either neomycin or tetracycline could be transferred to a recipient by the presence of -2 or -3 simultaneously in the donor. The presence of plasmids in 1030 was associated with a small increase in sensitivity to trimethoprim or sulphadiazine.


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