Effects of Streptomycin on the Growth and Sporulation of spp. Free

Abstract

Streptomycin inhibited growth and sporulation of species, although variations in sensitivity were recorded. In sporulating colonies, streptomycin increased both the length of the post-induction lag phase and the proportion of gemmae. Antibiotic concentrations (> 100 g ml) which resulted in the leakage of potassium ions from sporulating colonies also correlated with the condensation of hyphal cytoplasm into lysed segments. In vegetative colonies grown in > 500 g streptomycin ml, changes were seen in the organization of endoplasmic reticulum, mitochondria and nuclei. These inhibitory effects could be counteracted by the addition of calcium ions. Some observed effects were consistent with an impairment of normal mitochondrial function, but others, particularly in sporulating colonies, were possibly the result of interference with cellular functions regulated by calcium or other divalent cations.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-119-2-361
1980-08-01
2024-03-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/119/2/mic-119-2-361.html?itemId=/content/journal/micro/10.1099/00221287-119-2-361&mimeType=html&fmt=ahah

References

  1. Beakes G. W., Gay J. L. 1978; Light and electron microscopy of oospore maturation in Saprolegnia furcata. 1.Cytoplasmic changes.. Transactions of the British Mycological Society 71:11–24
    [Google Scholar]
  2. Boschetti A., Niggli V., Otz U., Wiedmer T. 1974; Resistance and sensitivity of chloroplast ribosomes to streptomycin in mutants of Chlamydomonas reinhardi.. Physiologia plantarum 31:169–174
    [Google Scholar]
  3. Cameron L. E., Léjohn H. B. 1972; On the involvement of calcium in amino acid transport and growth in the fungus Achlya.. Journal of Biological Chemistry 15:4729–4739
    [Google Scholar]
  4. Dexheimer J. 1966; Sur les modifications dureticulumendoplasmique des grains de pollen de Lobelia erinus (L.) traités par le chloramphenicol.. Comptes rendus hebdomadaire des séances de l’Académie des sciences D262:853–855
    [Google Scholar]
  5. Eckert S. W., Tsao P. H. 1962; A selective antibiotic medium for isolation of Phytophthora and Pythium from plant roots.. Phytopathology 52:771–777
    [Google Scholar]
  6. Elferink J. G. R. 1974; The effect of EDTA on yeast cell membranes.. Protoplasma 80:261–268
    [Google Scholar]
  7. Elliott C. G. 1972; Calcium chloride and growth and reproduction in Phytophthora cactorum.. Transactions of the British Mycological Society 58:169–172
    [Google Scholar]
  8. Finney D. J. 1971 Probit Analysis, 3rd edn.. Cambridge: Cambridge University Press.;
    [Google Scholar]
  9. Fletcher J. 1979; Ultrastructural investigation into the role of calcium on oosphere-initial development in Saprolegnia diclina.. Journal of General Microbiology 113:315–326
    [Google Scholar]
  10. Gale E. F., Cundliffe E., Reynolds P. E., Richmond H. M., Waring M. J. 1972 The Molecular Basis of Antibiotic Action pp. 278–379 London: John Wiley;
    [Google Scholar]
  11. Gay J. L., Greenwood A. D. 1966; Structural aspects of zoospore production in Saprolegnia ferax with particular reference to cell and vesicular membranes.. Colston Papers 18:95–110
    [Google Scholar]
  12. Gay J. L., Greenwood A. D., Heath I. B. 1971; The formation and behaviour of vacuoles (vesicles) during oosphere development and oospore germination in Saprolegnia.. Journal of General Microbiology 65:233–241
    [Google Scholar]
  13. Griffin D. H. 1966; Effect of electrolytes on differentiation in Achlya sp.. Plant Physiology 41:1254–1256
    [Google Scholar]
  14. Griffin D. H., Breuker C. 1969; Ribonucleic acid synthesis during the differentiation of sporangia in the water mould Achlya.. Journal of Bacteriology 98:689–696
    [Google Scholar]
  15. Griffin M. J., Coley-Smith J. R. 1971; Some effects of streptomycin on Pseudoperonospora humuli, the downy mildew of hop.. Journal of General Microbiology 69:117–134
    [Google Scholar]
  16. Griffin M. J., Coley-Smith J. R. 1975; Uptake of streptomycin by sporangia of Pseudoperonospora humuli and the inhibition of uptake by divalent metal cations.. Transactions of the British Mycological Society 65:265–278
    [Google Scholar]
  17. Heath I. B. 1975; The effect of antimicrotubule agents on the growth and ultrastructure of the fungus Saprolegnia ferax and their ineffectiveness in disrupting microtubules.. Protoplasma 85:147–176
    [Google Scholar]
  18. Heath I. B., Greenwood A. D. 1968; Electron microscopic observations of dividing somatic nuclei in Saprolegnia.. Journal of General Microbiology 53:287–289
    [Google Scholar]
  19. Heath I. B., Gay J. L., Greenwood A. D. 1971; Cell wall formation in the Saprolegniales: cytoplasmic vesicles underlying developing walls.. Journal of General Microbiology 65:225–232
    [Google Scholar]
  20. Hine R. B. 1962; Effect of streptomycin and pimaricin on growth and respiration of Pythium species.. Mycologia 54:640–646
    [Google Scholar]
  21. Lloyd D. 1974 The Mitochondria of Microorganisms. London: Academic Press;
    [Google Scholar]
  22. Marchant R., Smith D. G. 1968; The effect of chloramphenicol on growth and mitochondrial structure of Pythium ultimum.. Journal of General Microbiology 50:391–397
    [Google Scholar]
  23. May R. 1974; Microfilaments in yeast mitochondria.. Protoplasma 82:395–399
    [Google Scholar]
  24. Mcmeekin D. 1973; Streptomycin inhibition of Peronospora parasitica and its host reversed by manganese and calcium.. Phytopathology 63:34–36
    [Google Scholar]
  25. Mcmeekin D. 1978; Inhibition and stimulation of growth of Pythium by streptomycin.. Mycologia 70:880–883
    [Google Scholar]
  26. Olah J., Farkas J. 1978; Effect of temperature, pH, antibiotics, formalin and malachite green on the growth and survival of Saprolegnia and Achlyaparasitic on fish.. Aquaculture 13:273–288
    [Google Scholar]
  27. Olsen R. A. 1973; Triterpeneglycoides as inhibitors of fungal growth and metabolism. 5. Role of sterol contents of some fungi.. Physiologia plantarum 28:507–515
    [Google Scholar]
  28. Podbielkowska M., Zorska-Maciejewska B., Kacperska-Palacz A. 1975; Morphology of protoplast as affected by an inhibitor of respiration.. Protoplasma 82:201–208
    [Google Scholar]
  29. Shattock R. C., Shaw D. S. 1975; Mutants of Phytophthora infestans resistant to and dependent upon antibiotics.. Transactions of the British Mycological Society 64:29–41
    [Google Scholar]
  30. Shaw D. S., Elliott C. G. 1968; Streptomycin resistance and morphological variation in Phytophthora cactorum.. Journal of General Microbiology 51:75–84
    [Google Scholar]
  31. Trinci A. P. J., Gull K. 1970; Effect of actidione, griseofulvin and triphenyltin acetate on the kinetics of fungal growth.. Journal of General Microbiology 60:287–292
    [Google Scholar]
  32. Van Steveninck R. F. M. 1965; The significance of calcium on the apparent permeability of cell membranes and the effects of substitution with other divalent cations.. Physiologia plantarum 18:54–69
    [Google Scholar]
  33. Venis M. A. 1969; Streptomycin inhibition of protein synthesis in peas reversed by divalent cations.. Nature, London 221:1147–1148
    [Google Scholar]
  34. Vörös M. A. 1963; Mode of action of selective fungistatic effects of streptomycin.. Nature, London 199:1110–1111
    [Google Scholar]
  35. Vörös M. A. 1965; Streptomycin sensitivity of oomycetes due to the increased absorption of streptomycin by their mycelia.. Phytopathologische Zeitschrift 54:249–255
    [Google Scholar]
  36. Willoughby L. G., Pickering A. D. 1977; Viable Saprolegniaceae spores on the epidermis of the salmonid fish Salmo trutta and Salvelinus alpinus.. Transactions of the British Mycological Society 68:91–95
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-119-2-361
Loading
/content/journal/micro/10.1099/00221287-119-2-361
Loading

Data & Media loading...

Most cited Most Cited RSS feed