@article{mbs:/content/journal/micro/10.1099/00221287-119-2-297, author = "Waring, Richard B. and Scazzocchio, Claudio", title = "Nuclear and Mitochondrial Suppression of a Mitochondrially Inherited Cold-sensitive Mutation in Aspergillus nidulans", journal= "Microbiology", year = "1980", volume = "119", number = "2", pages = "297-311", doi = "https://doi.org/10.1099/00221287-119-2-297", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-119-2-297", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = "Partial suppressors of a mitochondrially inherited mutation, [cs-67], conferring cold-sensitivity at 20 0C were identified. These mapped at one mitochondrial and four unlinked nuclear loci. Most suppressors partially restored the cytochrome aa 3 deficiency of the cold-sensitive strain at 20 0C. Strains carrying two or more suppressors and [cs-67] showed considerably impaired growth. This effect was temperature-dependent, being more severe at 37 0C, and was not expressed in the presence of the [cs-67 +] allele. The cytochrome oxidase activity of one of these strains was no more heat-sensitive than that of the wild-type implying that these mutations did not directly modify cytochrome oxidase. The wild-type strain grown in the presence of chloramphenicol and the cold-sensitive strain grown at 20 0C had similar cytochrome spectra and mitochondrial membrane protein profiles on sodium dodecyl sulphate gradient acrylamide gels. [cs-67] conferred pleiotropically a low level of resistance to paromomycin at 37 0C. It is suggested that [cs-67] and the suppressors act at the level of the mitochondrial ribosome.", }