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N2 fixation was investigated in suspensions of washed cells of the photosynthetic bacterium Rhodopseudomonas capsulata grown on various carbon and nitrogen sources. Maximum nitrogenase activities were common to cultures supplied with glutamate. Cells grown with nitrate contained appreciable nitrogenase activity and produced H2 in the light. Nitrogenase activity in washed cells derived from various nutritional conditions was markedly increased when NaHCO3 was included in the reaction mixtures.
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