@article{mbs:/content/journal/micro/10.1099/00221287-119-1-273, author = "Matagne, R. F. and Vincenzotto, C.", title = "Isolation of Original Amino Acid, Vitamin and Carbon Source Mutants in the Green Alga Scenedesmus obliquus", journal= "Microbiology", year = "1980", volume = "119", number = "1", pages = "273-276", doi = "https://doi.org/10.1099/00221287-119-1-273", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-119-1-273", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", abstract = " Scenedesmus obliquus, grown under culture conditions which led to unicell populations, was treated with N-methyl-N′-nitro-N-nitrosoguanidine and then cells were plated on rich medium to recover auxotrophic mutants. Carbon source and vitamin (p-aminobenzoic acid, thiamin and nicotinamide)-requiring mutants were characterized. Amino acid-requiring mutants, some of types not previously isolated from green eukaryotes, were also obtained: 11 had a requirement for arginine, one for valine, two for threonine or isoleucine, and one for tryptophan, tyrosine or phenylalanine. The possible metabolic blocks of these amino acid-requiring mutants are discussed.", }