1887

Abstract

, grown under culture conditions which led to unicell populations, was treated with -methyl-′-nitro--nitrosoguanidine and then cells were plated on rich medium to recover auxotrophic mutants. Carbon source and vitamin (-aminobenzoic acid, thiamin and nicotinamide)-requiring mutants were characterized. Amino acid-requiring mutants, some of types not previously isolated from green eukaryotes, were also obtained: 11 had a requirement for arginine, one for valine, two for threonine or isoleucine, and one for tryptophan, tyrosine or phenylalanine. The possible metabolic blocks of these amino acid-requiring mutants are discussed.

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/content/journal/micro/10.1099/00221287-119-1-273
1980-07-01
2021-10-18
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