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Free-living cells of Rhizobium leguminosarum possess a constitutive succinate uptake system. Bacteroids isolated from nodules of Pisum sativum also showed immediate uptake of succinate. In both cases the uptake of [14C] succinate appeared to be dependent on an energized membrane. Fumarate, malate and several succinate analogues were also transported via the succinate system. One or both of the carboxyl groups of the succinate molecule must be free in order to be recognized by the carrier. Glucose had effects on the uptake or metabolism of succinate.
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