1887

Abstract

Cultures of KDHS2 reduced NO to NH with a concurrent increase in molar growth yield of 157% compared with fermentatively grown bacteria. The bacteria exhibited a (NO ) of 0·5 m and reduced NO-maximally at a rate of 01 mol h (mg dry wt). A partially purified nitrate reductase was obtained whichhad a (NO-) of 0·15 m. The reduction of NO to NH by resting bacteria was not inhibited by NH , glutamate, glutamine, methionine sulphoximine or azaserine. Glutamine synthetase affected neither the synthesis nor the activity of the NO -reducing enzymes. The results are consistent with the hypothesis that NO reduction to NH in this sp. is dissimilative. SO , but not SO , inhibited the reaction, apparently at the level of NO reduction.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-119-1-217
1980-07-01
2021-07-27
Loading full text...

Full text loading...

/deliver/fulltext/micro/119/1/mic-119-1-217.html?itemId=/content/journal/micro/10.1099/00221287-119-1-217&mimeType=html&fmt=ahah

References

  1. Bremner J. M. 1965; Inorganic forms of nitrogen.. In Methods of Soil Analysis, Part 2, Chemical and Microbiological Properties pp. 1179–1237 Edited by Black C. A. Madison, Wisconsin: American Society of Agronomy;
    [Google Scholar]
  2. Bremner J. M., Shaw K. 1958; Denitrification in soil. I. Methods of investigation.. Journal of Agricultural Science 51:22–39
    [Google Scholar]
  3. Brenchley J. E. 1973; Effects of methionine sulfoximine and methionine sulfone on glutamate synthesis in Klebsiella aerogenes.. Journal of Bacteriology 114:666–673
    [Google Scholar]
  4. Brenchley J. E., Prival M. J., Magasanik B. 1973; Regulation of the synthesis of enzymes responsible for glutamate formation in Klebsiella aerogenes.. Journal of Biological Chemistry 248:6122–6128
    [Google Scholar]
  5. Bryant M. P., Robinson I. M. 1961; An improved nonselective culture medium for ruminal bacteria and its use in determining diurnal variation in numbers of bacteria in the rumen.. Journal of Dairy Science 44:1446–1456
    [Google Scholar]
  6. Buresh R. J., Patrick W. H.JR 1978; Nitrate reduction to ammonium in anaerobic soil.. Soil Science Society of America Journal 42:913–918
    [Google Scholar]
  7. Caldwell D. E. 1974 Some relationships between physiology, morphology, and distribution in sulfur-oxidizing bacteria. Ph.D. thesis, Michigan State University, U.S.A.
    [Google Scholar]
  8. Caskey W. H., Tiedje J. M. 1979; Evidence for Clostridia as agents of dissimilatory reduction of nitrate to ammonium in soils.. Soil Science Society of America Journal 43:931–936
    [Google Scholar]
  9. Chiba S., Isiiimoto M. 1977; Studies on nitrate reductase of Clostridium perfringens. I. Purification, some properties, and effect of tungstate on its formation.. Journal of Biochemistry 82:1663–1671
    [Google Scholar]
  10. Cornish-Bowden A. 1976 Principles of Enzyme Kinetics. London: Butterworths;
    [Google Scholar]
  11. Deuel T. F., Stadtman E. R. 1970; Some kinetic properties of Bacillus subtilis glutamine synthetase.. Journal of Biological Chemistry 245:5206–5213
    [Google Scholar]
  12. Eisenthal R., Cornish-Bowden A. 1974; The direct linear plot. A new graphical procedure for estimating enzyme kinetic parameters.. Biochemical Journal 139:715–720
    [Google Scholar]
  13. Hasan S. M., Hall J. B. 1975; The physiological function of nitrate reduction in Clostridium perfringens.. Journal of General Microbiology 87:120–128
    [Google Scholar]
  14. Hasan M., Hall J. B. 1977; Dissimilatory nitrate reduction in Clostridium tertium.. Zeitschrift für allgemeine Mikrobiologie 17:501–506
    [Google Scholar]
  15. Hubbard J. S., Stadtman E. R. 1967; Regulation of glutamine synthetase. II. Patterns of feedback inhibition in microorganisms.. Journal of Bacteriology 93:1045–1055
    [Google Scholar]
  16. Inderlied C. B., Delwiche E. A. 1973; Nitrate reduction and the growth of Veillonella alcalescens.. Journal of Bacteriology 114:1206–1212
    [Google Scholar]
  17. Keeney D. R., Chen R. L., Graetz D. A. 1971; Importance of denitrification and nitrate reduction in sediments to the nitrogen budgets in lakes.. Nature, London 233:66
    [Google Scholar]
  18. Kemp J. D., Atkinson D. E., Ehret A., Lazzarini R. A. 1963; Evidence for the identity of the nicotinamide adenine dinucleotide phosphate-specific sulfite and nitrite reductases of Escherichia coli.. Journal of Biological Chemistry 238:3466–3471
    [Google Scholar]
  19. Koike I., Hattori A. 1978; Denitrification and ammonia formation in anaerobic coastal sediments.. Applied and Environmental Microbiology 35:278–282
    [Google Scholar]
  20. Macrae D.C, Ancajas R. R., Salandanan S. 1968; The fate of nitrate nitrogen in some tropical soils following submergence.. Soil Science 105:327–334
    [Google Scholar]
  21. Newman B. M., Cole J. A. 1977; Lack of a regulatory function for glutamine synthetase protein in the synthesis of glutamate dehydrogenase and nitrite reductase in Escherichia coli K12.. Journal of General Microbiology 98:369–377
    [Google Scholar]
  22. Nommik H. 1956; Investigations on denitrification in soils.. Acta agriculturae scandinavica 6:197–228
    [Google Scholar]
  23. Prabhakararao K., Nicholas D. J. D. 1970; The reduction of sulphite, nitrite, and hydroxyl-amine by an enzyme from baker’s yeast.. Biochimica et biophysica acta 216:122–129
    [Google Scholar]
  24. Sørensen J. 1978; Capacity for denitrification and reduction of nitrate to ammonia in a coastal marine sediment.. Applied and Environmental Microbiology 35:301–305
    [Google Scholar]
  25. Stanford G., Legg J. O., Dzienia S., Simpson E. C.JR 1975a; Denitrification and associated nitrogen transformations in soils.. Soil Science 120:147–152
    [Google Scholar]
  26. Stanford G., Legg J. O., Stanley T. E. 1975b; Fate of 15N-labelled nitrate in soils under anaerobic conditions.. In Proceedings of the Second International Conference on Stable Isotopes pp. 667–673 Edited by Klein E. R., Klein P. D. Oak Brook, III.: National Technical Information Service;
    [Google Scholar]
  27. Tiedje J. M., Firestone R. B., Firestone M. K., Betlach M. R., Smith M. S., Caskey W. H. 1979; Methods for the production and use of 18N in studies of denitrification.. Soil Science Society of America Journal 43:709–715
    [Google Scholar]
  28. Tubb R. S. 1974; Glutamine synthetase and ammonium regulation of nitrogenase synthesis in Klebsiella.. Nature, London 251:481–484
    [Google Scholar]
  29. Wijler J., Delwiche C. C. 1954; Investigations on the denitrifying process in soil.. Plant and Soil 5:155–169
    [Google Scholar]
  30. Yordy D. M., Delwiche E. A. 1979; Nitrite reduction in Veillonella alcalescens.. Journal of Bacteriology 137:905–911
    [Google Scholar]
  31. Zalkin H. 1973; Anthranilate synthetase.. Advances in Enzymology 38:1–39
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-119-1-217
Loading
/content/journal/micro/10.1099/00221287-119-1-217
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error