1887

Abstract

A mucopolysaccharidase derived from a pathogenic strain of was isolated and purified by fractionation with cold acetone and ion-exchange chromatography on DEAE-cellulose, pH 8·0. Three detectable enzyme activities from concentrated supernatant filtrates were obtained in a fraction precipitated by three volumes of cold acetone; these were DNAase, hyaluronidase and chondroitinase-like activity. Separation of the DNAase was achieved by ion-exchange chromatography. Fractions designated as purified mucopolysaccharidase contained both hyaluronidase and chondroitinase-like activity.

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1980-07-01
2024-04-18
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References

  1. Cheng K. J., Costerton J. W. 1973; Localization of alkaline phosphatase in three Gramnegative rumen bacteria.. Journal of Bacteriology 116:424–440
    [Google Scholar]
  2. Felner J. M., Dowell V. R. 1971; Bacteroides bacteremia.. American Journal of Medicine 50:787–796
    [Google Scholar]
  3. Finegold S. M., Rosenblatt J. E. 1973; Practical aspects of anaerobic sepsis.. Medicine 52:311–322
    [Google Scholar]
  4. Finegold S. M., Marsh V. H., Bartlett J. G. 1971; Anaerobic infections in the compromised host.. In Proceedings of the International Conference on Nosocomial Infections pp. 123–134 Edited by Brachman P. S., Eickhoff T. C. Baltimore: Waverly Press;
    [Google Scholar]
  5. Gibbons R. J., Macdonald J. B. 1961; Degra-dation of collagenous substrates by Bacteroides melaninogenicus.. Journal of Bacteriology 81:614–621
    [Google Scholar]
  6. Hahn L. 1947; Uber die enzymatische Spaltung der Hyaluronsaure.. Biochemische Zeitschrift 318:123–137
    [Google Scholar]
  7. Kass E. H., Seastone C. V. 1944; The role of mucoid polysaccharide (hyaluronic acid) in the virulence of Group A hemolytic streptococci.. Journal of Experimental Medicine 79:319–329
    [Google Scholar]
  8. Levinson M. E. 1973; The importance of anaerobic bacteria in infectious diseases.. Medical Clinics of North America 57:1015–1027
    [Google Scholar]
  9. Murphy R. A. 1972; Improved antihyaluronidase test applicable to the microtitration technique.. Applied Microbiology 23:1170–1171
    [Google Scholar]
  10. Ornstein L. 1964; Disc electrophoresis.. Annals of the New York Academy of Sciences 121:321–349
    [Google Scholar]
  11. Porshen R. K., Sonntag S. 1974; Extracellular deoxyribonuclease production by anaerobic bacteria.. Applied Microbiology 27:1031–1033
    [Google Scholar]
  12. Porshen R. K., Spaulding E. H. 1974; Phosphatase activity of anaerobic organisms.. Applied Microbiology 27:744–747
    [Google Scholar]
  13. Rein J. M., Cosman B. 1971; Bacteroides necrotizing fasciitis of the upper extremity.. Plastic and Reconstructive Surgery 48:592–594
    [Google Scholar]
  14. Rudek W., Haque R. 1976; Extracellular enzymes of the genus Bacteroides.. Journal of Clinical Microbiology 4:458–460
    [Google Scholar]
  15. Sallman B., Birleland J. M. 1950; The role of hyaluronidase in hemolytic streptococcal infection.. Annals of the New York Academy of Sciences 52:1062–1069
    [Google Scholar]
  16. Salyers A. A., Vercellotti J. R., West S. E. H., Wilkins T. D. 1977; Fermentation of mucin and plant polysaccharides by strains of Bacteroides from the human colon.. Applied and Environmental Microbiology 33:319–322
    [Google Scholar]
  17. Smith R. E., Willett N. P. 1968; Rapid plate method for screening hyaluronidase and chondroitin sulfatase-producing microorganisms.. Applied Microbiology 16:1434–1436
    [Google Scholar]
  18. Sober H. A., Gutter F. J., Wyckoff M. M., Peterson E. A. 1956; Chromatography of proteins. II. Fractionation of serum protein on aminoexchange cellulose.. Journal of the American Chemical Society 78:756–761
    [Google Scholar]
  19. Steffen E. K., Hentges D. J., Campbell B. S. 1979; Selected enzymes of clinical anaerobic isolates.. Abstracts of the Annual Meeting of the American Society for Microbiology B22 p. 19 Washington D.C.: American Society for Microbiology;
    [Google Scholar]
  20. Thurston C. F., Hardingham T. E., Muir H. 1975; The kinetics of degradation of chondroitin sulphates and hyaluronic acid by chondroitinase from Proteus vulgaris.. Biochemical Journal 145:397–400
    [Google Scholar]
  21. Walker C. B., Wilkins T. D. 1976; Use of a semisolid agar for initiation of pure Bacteroides fragilis infection in mice.. Infection and Immunity 14:721–725
    [Google Scholar]
  22. Weiss C. 1937; Observations on Bacteroides melaninogenicus. Demonstration of fibrinolysin, pathogenicity and serological types.. Proceedings of the Society for Experimental Biology 37473–476
    [Google Scholar]
  23. Willis A. T., Young S. E. J., Ferguson I. R. 1974; Infections due to non-sporing anaerobes: some illustrative cases.. In Infection with Non-Sporing Anaerobic Bacteria pp. 189–201 Edited by Philips I., Sussman M. Edinburgh: Churchill Livingstone;
    [Google Scholar]
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