1887

Abstract

A mucopolysaccharidase derived from a pathogenic strain of was isolated and purified by fractionation with cold acetone and ion-exchange chromatography on DEAE-cellulose, pH 8·0. Three detectable enzyme activities from concentrated supernatant filtrates were obtained in a fraction precipitated by three volumes of cold acetone; these were DNAase, hyaluronidase and chondroitinase-like activity. Separation of the DNAase was achieved by ion-exchange chromatography. Fractions designated as purified mucopolysaccharidase contained both hyaluronidase and chondroitinase-like activity.

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/content/journal/micro/10.1099/00221287-119-1-211
1980-07-01
2021-08-04
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