The group G antigen of lactobacilli was identified as a negatively-charged cell wall polysaccharide. The components of the preparation isolated from cell walls of L. salivarius subsp. salivarius by mild acid hydrolysis were glucose, galactose and lesser amounts of rhamnose, N-acetylglucosamine and phosphate. Quantitative serological studies on acid-released polysaccharide and enzymic lysates of cell walls showed that rhamnose was the immunodominant component. The antigen was also detected in L. salivarius subsp. salicinus but not in a recent isolate of L. salivarius, strain IV CL-37.
CampbellL. K., KnoxK. W., WickenA. J.1978; Extractability of cell wall polysaccharide from lactobacilli and streptococci by autoclaving and by dilute acid.. Infection and Immunity 22:842–851
DischeZ., ShettlesL. B.1948; A specific color reaction of methylpentoses and a spectrophotometric micromethod for their determination.. Journal of Biological Chemistry 175:595–603
FitzgeraldR. J., FitzgeraldD. B., AdamsB. O., DuanyL. F.1980; Cariogenicity of human oral lactobacilli in hamsters.. Journal of Dental Research 59:832–837
HallE. A., KnoxK. W.1965; Properties of the polysaccharide and mucopeptide components of the cell wall of Lactobacillus casei.. Biochemical Journal 96:310–318
HuggettA. ST. G., NixonD. A.1957; Use of glucose oxidase, peroxidase and o-dianisidine in determination of blood and urinary glucose.. Lancet ii:368–370
JacquesN. A., BrownR. A., SharpeM. E., FitzgeraldR. J.1980; Characterization of two strains of cariogenic lactobacilli.. Journal of General Microbiology 118:283–286
KnoxK. W., HewettM. J., WickenA. J.1970; Studies on the group F antigen of lactobacilli: antigenicity and serological specificity of teichoic acid preparations.. Journal of General Microbiology 60:303–313
KnoxK. W., JacquesN. A., CampbellL. K., WickenA. J., HurstS. F., BleiweisA. S.1979; Phenotypic stability of the cell wall of Streptococcus mutans Ingbritt grown under varying conditions.. Infection and Immunity 26:1071–1078
RogosaM., WisemanR. F., MitchellJ. A., DisraelyM. N., BeananA. J.1953; Species differentiation of oral lactobacilli from man including descriptions of Lactobacillus salivarius nov.spec. and Lactobacillus cellobiosus nov.spec.. Journal of Bacteriology 65:681–699
SharpeM. E.1970; Cell wall and cell membrane antigens used in the classification of lactobacilli.. International Journal of Systematic Bacteriology 20:509–518
ShimohashiH., KodairaS., SuegaraN.1976; Grouping antigens of four Lactobacillus species and their characteristics.. Japanese Journal of Microbiology 20:405–413
StromingerJ. L., ParkJ. T., ThompsonR. E.1959; Composition of the cell wall of Staphylococcus aureus: its relation to the mechanism of action of penicillin.. Journal of Biological Chemistry 234:3263–3268
WallenfelsK., KurzG.1962; Über die Spezifität der Galaktosedehydrogenase aus Pseudomonas saccharophila und deren Anwendung als analytisches Hilfsmittel.. Biochemische Zeitschrift 335:559–567
WickenA. J., KnoxK. W.1970; Studies on the group F antigen of lactobacilli: isolation of a teichoic acid-lipid complex from Lactobacillus fermenti NCTC 6991.. Journal of General Microbiology 60:293–301
WickenA. J., GibbensJ. W., KnoxK. W.1973; Comparative studies on the isolation of membrane lipoteichoic acid from Lactobacillus fermenti.. Journal of Bacteriology 113:365–372
YadomaeT., OhnoN., MiyazakiT.1979; On the phosphate linkages and the structure of a disaccharide unit of the type-specific polysaccharide of pneumococcus type XIX.. Carbohydrate Research 75:191–198
YokogawaK., KawataS., TakemuraT., YoshimuraY.1975; Purification and properties of lytic enzymes from Streptomyces globisporus 1829.. Agricultural and Biological Chemistry 39:1533–1543