Nine strains of Bacillus mycoides, 10 of Bacillus cereus and 18 of Bacillus thuringiensis were analysed using pyrolysis gasliquid chromatography. Application of canonical variates analysis to the resultant pyrograms showed that these organisms formed three distinct groups. Stepwise discriminant analysis with jacknifing showed that of the original 27 peak heights used to define the canonical variates axes, only two were needed to obtain reasonably stable discriminant functions. These discriminant functions (Dixon, 1975) are potentially valuable in identification. Jacknifing also indicated that, had it been an unknown, one strain of B. mycoides would have been incorrectly identified as B. cereus.
MacfieH. J. H.,
GutteridgeC. S.1978; Analysis of pyrolysis gas-liquid chromatography data using multivariate statistical techniques.. Journal of Applied Bacteriology 45:iv–v
MacfieH. J. H.,
GutteridgeC. S.,
NorrisJ. R.1978; Use of canonical variates analysis in differentiation of bacteria by pyrolysis gas-liquid chromatography.. Journal of General Microbiology 104:67–74
O’DonnellA. G.,
NorrisJ. R.1980; Pyrolysis gas-liquid chromatographic studies in the genus Bacillus.. In Classification and Identification of the Aerobic Endospore-forming Bacteria. Edited by BerkeleyR. C. W.,
GoodfellowM.
London: Academic Press; (in the Press)
O’DonnellA. G.,
NorrisJ. R.,
BerkeleyR. C. W.,
ClausD.,
KanekoT.,
LoganN. A.,
NozakiR.1980; Characterisation of Bacillus subtilis, Bacillus pumilus, Bacillus licheniformis and Bacillus amyloliquefaciens by pyrolysis gas-liquid chromatography: characterisation tested using DNA-DNA hybridisation; biochemical tests and API systems.. International Journal of Systematic Bacteriology (in the Press)
OxborrowG. S.,
FieldsN. D.,
PuleoJ. R.1976; Preparation of pure microbiological samples for pyrolysis gas-liquid chromatography studies.. Applied and Environmental Microbiology 32:306–309
OxborrowG. S.,
FieldsN. D.,
PuleoJ. R.1977a; Pyrolysis gas-liquid chromatography studies of the genus Bacillus. Effect of growth time on pyrochromatogram reproducibility.. In Analytical Pyrolysis pp. 69–76 Edited by JonesC. E. R.,
CramersC. A.
Amsterdam: Elsevier Scientific Publishing Co.;
OxborrowG. S.,
FieldsN. D.,
PuleoJ. R.1977b; Pyrolysis gas-liquid chromatography of the genus Bacillus: effect of growth media on pyrochromatogram reproducibility.. Applied and Environmental Microbiology 33:865–870
StackM. V.,
DonoghueH. D.,
TylerJ. E.,
MarshallM.1977; Comparison of oral streptococci by pyrolysis gas-liquid chromatography.. In Analytical Pyrolysis pp. 57–68 Edited by JonesC. E. R.,
CramersC. A.
Amsterdam: Elsevier Scientific Publishing Co.;