1887

Abstract

Several and species chosen at random and solubilized with sodium dodecyl sulphate showed a common periodic acidSchiff positive band with an apparent molecular weight of about 64000, when examined by polyacrylamide gel electrophoresis. Another more cathodic minor band was detected in and . The common periodic acid-Schiff positive band appeared when a precipitate of serum constituents of the uninoculated growth medium after incubation was examined. The minor band was identified as a serum glycoprotein contaminating mycoplasmas grown in the presence of swine serum. We draw attention to the compounds as a possible source of error in serological tests or in the lymphocyte stimulation response. After lithium diiodosalicylate solubilization and aqueous phenol extraction, polyacrylamide gel electrophoresis showed a periodic acidSchiff positive band in membranes from (molecular weight 75000) and (molecular weight 80000), suggesting the presence of a membrane glycoprotein. Such a glycoprotein was absent from . Since the common periodic acidSchiff positive band was not extracted by aqueous phenol, this growth medium constituent did not contaminate the preparations of membrane glycoproteins. However, the minor band was present in glycoprotein preparations of grown in the presence of swine serum.

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1980-07-01
2024-04-23
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References

  1. Bannerman E., Nicolet J. 1971; Isolation and identification of porcine mycoplasma in Switzerland.. Schweizer Archiv für Tierheilkunde 113:697–710
    [Google Scholar]
  2. Bartlett R. 1958; Phosphorus assay in column chromatography.. Journal of Biological Chemistry 234:466–471
    [Google Scholar]
  3. Bradbury J. M., Jordan F. T. W. 1971; The adsorption of gamma-globulins to Mycoplasma gallisepticum and the possible role in non-specific serological reactions.. Veterinary Record 89:318
    [Google Scholar]
  4. Bradbury J. M., Jordan F. T. W. 1972; Studies on the adsorption of certain medium proteins to Mycoplasma gallisepticum and their influence on agglutination and haemagglutination.. Journal of Hygiene 70:267–278
    [Google Scholar]
  5. Chrambach A., Reisfeld R. A., Wyckoff M., Zaccari J. 1967; A procedure for rapid and sensitive staining of protein fractionated by polyacrylamide gel electrophoresis.. Analytical Biochemistry 20:150–154
    [Google Scholar]
  6. Clark H. W., Baily J. S., Mcbrown T. 1969; Variations on mycoplasma antigenic activity.. In Mycoplasma Diseases of Man Proceedings of an International Symposium, Reinhardsbrunn Castle. Sprossig M., Witzleb W. Edited by Jena:: Gustav Fischer.;
    [Google Scholar]
  7. Friis N. F. 1971; Mycoplasmas cultivated from the respiratory tract of Danish pigs.. Acta veterinaria scandinavica 12:69–79
    [Google Scholar]
  8. Goel M. C., Lemcke R. M. 1975; Dissociation of Mycoplasma gallisepticum membranes with lithium diiodosalicylate and isolation of a glycoprotein.. Annales de Microbiologie 126B:299–312
    [Google Scholar]
  9. Goodwin R. F. W., Pomeroy A. P., Whittle-Stone P. 1965; Production of enzootic pneumonia in pigs with a mycoplasma.. Veterinary Record 77:1247–1249
    [Google Scholar]
  10. Hartree E. F. 1972; Determination of protein; a modification of the Lowry method that gives a linear photometric response.. Analytical Biochemistry 48:422–427
    [Google Scholar]
  11. Hollingdale M. R., Lemcke R. M. 1972; Membrane antigens of Mycoplasma hominis.. Journal of Hygiene 70:85–98
    [Google Scholar]
  12. Johansson K. E. 1978; Production and utilization of monospecific antisera against membrane proteins from Acholeplasma laidlawii.. Zentralblatt für Bakteriologie, Parasitenkunde, Infektions- krankheiten und Hygiene (Abteilung I, Originate A) 241:199–200
    [Google Scholar]
  13. Jordan F. T. W., Kulasegaram P. 1968; Non-specific antibodies in chickens inoculated intratracheally with Mycoplasma gallisepticum.. Journal of Comparative Pathology 78:407
    [Google Scholar]
  14. Kahane I., Brunner H. 1977; Isolation of a glycoprotein from Mycoplasma pneumoniae membranes.. Infection and Immunity 18:273–277
    [Google Scholar]
  15. Kahane I., Marchesi V. T. 1973; Studies on the orientation of proteins in mycoplasma and erythrocyte membranes.. Annals of the New York Academy of Sciences 225:38–45
    [Google Scholar]
  16. Kapitany R. A., Zebrowski E. J. 1973; A high resolution PAS stain for polyacrylamide gel electrophoresis.. Analytical Biochemistry 56:361–369
    [Google Scholar]
  17. Lazary S., Rivera E., De Weck A. L., Gerber H., Nicolet J. 1974; In vitro stimulation of bovine leucocytes by phytohaemagglutinin and other mitogens.. Research in Veterinary Science 17:344–350
    [Google Scholar]
  18. Marchesi V. T., Andrews E. P. 1971; Glycoproteins: isolation from cell membranes with lithium diiodosalicylate.. Science 174:1247–1248
    [Google Scholar]
  19. Morowitz H. J., Terry T. M. 1969; Characterization of the plasma membrane of Mycoplasma laidlawii.. Biochimica et biophysica acta 183:276–294
    [Google Scholar]
  20. Ouchterlony O. 1967; Immunodiffusion and immunoelectrophoresis.. In Handbook of Experimental Immunology pp. 655–706 Weir D. M. Edited by Oxford:: Blackwell Scientific Publications.;
    [Google Scholar]
  21. Paroz PH., Nicolet J. 1978; Glycoproteins in mycoplasmas. Contamination with serum aggregates from growth media.. Experientia 34:1668
    [Google Scholar]
  22. Paroz PH., Krawinkler M., Nicolet J. 1976; Verwendung der Polyacrylamid-Gel-Elektrophorese in der Diagnostik von Nutztier-Mycoplasmen.. Zentralblatt für Veterinärmedizin B24:668–677
    [Google Scholar]
  23. Razin S., Rottem S. 1976; Techniques for the manipulation of mycoplasma membranes.. In Biochemical Analysis of Membranes pp. 3–26 Maddy A. H. Edited by London:: Chapman and Hall.;
    [Google Scholar]
  24. Rottem S., Hasin M., Razin S. 1973; Binding of proteins to mycoplasma membranes.. Biochimica et biophysica acta 298:876–886
    [Google Scholar]
  25. Schiefer H. G., Gerhardt U., Brunner H., Krupe M. 1974; Studies with lectins on the surface carbohydrate structure of mycoplasma membranes.. Journal of Bacteriology 120:81–88
    [Google Scholar]
  26. SchÜtten H. J., Mogensen H. H., Lind K. 1978; Mycoplasma pneumoniae antigens demonstrated by crossed immunoelectrophoresis.. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I, Originate A) 241:240
    [Google Scholar]
  27. Troy A., Scott J., Melvin E. 1953; Determination of dextran with anthrone.. Analytical Chemistry 25:1656–1661
    [Google Scholar]
  28. Williams M. H., Brostoff J., Roitt J. M. 1970; Possible role of Mycoplasma fermentans in pathogenesis of rheumatoid arthritis.. Lancet 2:277–288
    [Google Scholar]
  29. Zacharius R. M., Zell T. E., Morrison J. H., Woodlock J. J. 1969; Glycoprotein staining following electrophoresis on acrylamide gels.. Analytical Biochemistry 30:148–152
    [Google Scholar]
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