1887

Abstract

Several and species chosen at random and solubilized with sodium dodecyl sulphate showed a common periodic acidSchiff positive band with an apparent molecular weight of about 64000, when examined by polyacrylamide gel electrophoresis. Another more cathodic minor band was detected in and . The common periodic acid-Schiff positive band appeared when a precipitate of serum constituents of the uninoculated growth medium after incubation was examined. The minor band was identified as a serum glycoprotein contaminating mycoplasmas grown in the presence of swine serum. We draw attention to the compounds as a possible source of error in serological tests or in the lymphocyte stimulation response. After lithium diiodosalicylate solubilization and aqueous phenol extraction, polyacrylamide gel electrophoresis showed a periodic acidSchiff positive band in membranes from (molecular weight 75000) and (molecular weight 80000), suggesting the presence of a membrane glycoprotein. Such a glycoprotein was absent from . Since the common periodic acidSchiff positive band was not extracted by aqueous phenol, this growth medium constituent did not contaminate the preparations of membrane glycoproteins. However, the minor band was present in glycoprotein preparations of grown in the presence of swine serum.

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1980-07-01
2021-10-26
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