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A mutant having a 30-fold increased ability to synthesize pyrrolnitrin was isolated from Pseudomonas aureofaciens ATCC 15926 after mutagenesis with N-methyl-N′-nitro-N-nitrosoguanidine. The mutant strain also differed from the parent strain in pigmentation and in its inability to catabolize anthranilic acid and the benzene moiety of tryptophan and kynurenine.
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