The specialized transducing phage λ (Borck , 1976) was found to carry about 5 kilobases of DNA. It was shown to have an intact gene but none of the known neighbouring genetic loci. The phage (which is known to be deficient in its site-specific recombination functions) was shown to integrate into the chromosome of bacterial recipients at the locus. Excision from this site occasionally generated recombinant phages that had exchanged their allele for the one originally present in the host. In this way λ derivatives were prepared from lysogens of two strains carrying the amber mutations and ; these phages were proved by several tests to contain the expected amber mutations.


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