1887

Abstract

Duplexes were prepared between C-labelled rRNA from both C7 and N54 and DNA from 16 actinomycetes representing the genera and . The relationships between the organisms were determined by plotting the temperature at which 50 % of the duplex was denatured (T (e)) against the percentage of rRNA binding (g C-labelled rRNA duplexed per 100 g filter-bound DNA). All of the strains formed stable duplexes but each organism occupied a definite area on the rRNA similarity map. All of the organisms share a close phylogenetic relationship but representatives of the genera and fell into four recognizable clusters on the similarity map. These data support and extend current trends in the classification of and allied taxa. The guanine plus cytosine content of the DNA from the test strains was within the range 693 to 769 mol %.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-118-2-313
1980-06-01
2021-05-12
Loading full text...

Full text loading...

/deliver/fulltext/micro/118/2/mic-118-2-313.html?itemId=/content/journal/micro/10.1099/00221287-118-2-313&mimeType=html&fmt=ahah

References

  1. Bradley S. G., Mordarski M. 1976; Association of polydeoxyribonucleotides of deoxyribonucleic acids from nocardioform bacteria.. In The Biology of the Nocardiae pp. 310–336 Edited by Goodfellow M., Brownell G. H., Serrano J. A. London: Academic Press;
    [Google Scholar]
  2. Burton K. 1956; A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.. Biochemical Journal 62:315–323
    [Google Scholar]
  3. Crombach W. H. J. 1978; DNA base ratios and DNA hybridisation studies of coryneform bacteria, mycobacteria and nocardiae.. In Coryneform Bacteria pp. 161–179 Edited by Bousfield I. J., Callely A. G. London: Academic Press;
    [Google Scholar]
  4. De Ley J., De Smedt J. 1975; Improvements of the membrane filter method for DNA:rRNA hybridization.. Antonie van Leeuwenhoek 41:287–307
    [Google Scholar]
  5. De Smedt J., De Ley J. 1977; Intra- and intergeneric similarities of Agrobacterium ribo- somal ribonucleic acid cistrons.. International Journal of Systematic Bacteriology 27:222–240
    [Google Scholar]
  6. Doi R. H., Igarashi R. T. 1965; Conservation of ribosomal and messenger ribonucleic acid cistrons in Bacillus species.. Journal of Bacteriology 90:384–390
    [Google Scholar]
  7. Dubnau D., Smith L, Morell P., Marmur J. 1965; Gene conservation in Bacillus species. I. Conserved genetic and nucleic acid sequence homologies.. Proceedings of the National Academy of Sciences of the United States of America 54491–498
    [Google Scholar]
  8. Goodfellow M. 1971; Numerical taxonomy of some nocardioform bacteria.. Journal of General Microbiology 69:33–80
    [Google Scholar]
  9. Goodfellow M., Alderson G. 1977; The actinomycete genus Rhodococcus: a home for the ‘ rhodochrous’ complex.. Journal of General Microbiology 100:99–122
    [Google Scholar]
  10. Goodfellow M., Minnikin D. E. 1977; Nocardioform bacteria.. Annual Review of Microbiology 31:159–180
    [Google Scholar]
  11. Goodfellow M., Minnikin D. E. 1978; Numerical and chemical methods in the classification of Nocardia and related taxa.. Zentralblatt für Bakteriologie, Parasitenkunde, Infektionskrank- heiten und Hygiene (Abteilung I) Supplement 6:43–51
    [Google Scholar]
  12. Goodfellow M., Orlean P. A. B., Collins M. D., Alshamaony L., Minnikin D. E. 1978; Chemical and numerical taxonomy of strains received as Gordona aurantiaca. . Journal of General Microbiology 109:57–68
    [Google Scholar]
  13. Johnson J. L., Francis B. S. 1975; Taxonomy of the Clostridia: ribosomal ribonucleic acid homologies among the species.. Journal of General Microbiology 88:229–244
    [Google Scholar]
  14. Johnson J. L., Anderson R.S, Ordal E. J. 1970; Nucleic acid homologies among oxidasenegative Moraxella species.. Journal of Bacteriology 101:568–573
    [Google Scholar]
  15. Jones D. 1975; A numerical taxonomic study of coryneform and related bacteria.. Journal of General Microbiology 87:52–96
    [Google Scholar]
  16. Jones D. 1978; An evaluation of the contributions of numerical taxonomic studies to the classification of coryneform bacteria.. In Coryneform Bacteria pp. 13–46 Edited by Bousfield I. J., Callely A. G. London: Academic Press;
    [Google Scholar]
  17. Keddie R. M., Cure G. L. 1978; Cell wall composition of coryneform bacteria.. In Coryneform Bacteria pp. 47–83 Edited by Bousfield I. J., Callely A. G. London: Academic Press;
    [Google Scholar]
  18. Lechevalier M. P., Lechevalier H. A. 1970; Chemical composition as a criterion in the classification of aerobic actinomycetes.. International Journal of Systematic Bacteriology 20:435–444
    [Google Scholar]
  19. Mcconkey E. H. 1967; The fractionation of RNA’s by sucrose gradient centrifugation.. Methods in Enzymology 12:620–634
    [Google Scholar]
  20. Marmur J., Doty P. 1962; Determination of base composition of deoxyribonucleic acid from its thermal denaturation temperature.. Journal of Molecular Biology 5:109–118
    [Google Scholar]
  21. Meys W. H., Schilperoort R. A. 1971; Determination of the amount of DNA on nitrocellulose membrane filters.. FEBS Letters 12:166–168
    [Google Scholar]
  22. Minnikin D. E., Goodfellow M. 1976; Lipid composition in the classification and identification of nocardiae and related taxa.. In The Biology of the Nocardiae pp. 160–219 Edited by Goodfellow M., Brownell G. H., Serrano J. A. London: Academic Press;
    [Google Scholar]
  23. Minnikin D. E., Goodfellow M. 1980; Lipid composition in the classification and identification of acid fast bacteria.. In Microbiological Classification and Identification pp. 189–256 Edited by Goodfellow M., Board R. G. London: Academic Press;
    [Google Scholar]
  24. Minnikin D. E., Goodfellow M., Collins M. D. 1978; Lipid composition in the classification and identification of coryneform and related taxa.. In Coryneform Bacteria pp. 85–160 Edited by Bousfield T. J., Callely A. G. London: Academic Press;
    [Google Scholar]
  25. Moore R. L., Mccarthy B. J. 1967; Comparative study of ribosomal ribonucleic acid cistrons in enterobacteria and myxobacteria.. Journal of Bacteriology 94:1066–1074
    [Google Scholar]
  26. Moore R. L., Mccarthy B. J. 1969; Base sequence homology and renaturation studies of the deoxyribonucleic acid of extremely halophilic bacteria.. Journal of Bacteriology 99:255–262
    [Google Scholar]
  27. Mordarski M., Szyba K., Pdlverer G., Goodfellow M. 1976; Deoxyribonucleic acid reassociation in the classification of the ‘rhodo- chrous’ complex and allied taxa.. Journal of General Microbiology 94:235–245
    [Google Scholar]
  28. Mordarski M., Goodfellow M., Szyba K., Pulverer G., Tkacz A. 1977; Classification of the ‘rhodochrous’ complex and allied taxa based upon deoxyribonucleic acid reassociation.. International Journal of Systematic Bacteriology 27:31–37
    [Google Scholar]
  29. Mordarski M., Goodfellow M., Szyba K., Pulverer G., Tkacz A. 1978; Deoxyribonucleic acid base composition and homology studies on Rhodococcus and allied taxa.. Zentrcilblatt für Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene (Abteilung I) Supplement 6:99–106
    [Google Scholar]
  30. Mordarski M., Goodfellow M., Szyba K., Tkacz A., Pulverer G., Schaal K. P. 1980; Deoxyribonucleic acid reassociation in the classification of the genus Rhodococcus. . International Journal of Systematic Bacteriology in the Press
    [Google Scholar]
  31. Pace B., Campbell L. L. 1971a; Homology of ribosomal ribonucleic acid of Desulfovibrio species with Desulfovibrio vulgaris. . Journal of Bacteriology 106:717–719
    [Google Scholar]
  32. Pace B., Campbell L. L. 1971b; Homology of ribosomal ribonucleic acid of diverse bacterial species with Escherichia coli and Bacillus stearo- thermophilus. . Journal of Bacteriology 107:543–547
    [Google Scholar]
  33. Palleroni N. J., Kunisawa R., Contcpoulou R., Doudoroff M. 1973; Nucleic acid homologies in the genus Pseudomonas. . International Journal of Systematic Bacteriology 23:333–339
    [Google Scholar]
  34. Rowbotham T. J., Cross T. 1977; Rhodococcus coprophilus sp.nov.: an aerobic nocardioform actinomycete belonging to the ‘rhodochrous’ complex.. Journal of General Microbiology 100:123–138
    [Google Scholar]
  35. Saito H., Miura K. I. 1963; Preparation of transforming deoxyribonucleic acid by phenol treatment.. Biochimica et biophysica acta 72:619–629
    [Google Scholar]
  36. Shirling E. B., Gottlieb D. 1968; Cooperative description of type cultures of Streptomyces. II. Species descriptions from first study.. International Journal of Systematic Bacteriology 18:69–189
    [Google Scholar]
  37. Shirling E. B., Gottlieb D. 1972; Cooperative description of type strains of Streptomyces. V. Additional descriptions.. International Journal of Systematic Bacteriology 22:265–394
    [Google Scholar]
  38. Takahashi H., Saito H., Ikeda Y. 1967; Species specificity of the ribosomal RNA cistrons in bacteria.. Biochimica et biophysica acta 134:124–133
    [Google Scholar]
  39. Yamada K., Komagata K. 1970; Taxonomic studies on coryneform bacteria. III. DNA base composition of coryneform bacteria.. Journal of General and Applied Microbiology 16:215–224
    [Google Scholar]
  40. Zopf W. 1891; Cber Ausscheidung von Fettfarb- stoffen (Lipochromen) seitens gewisser Spaltpilze.. Berichte der Deutschen botanischen Gesellschaft 9:22–28
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-118-2-313
Loading
/content/journal/micro/10.1099/00221287-118-2-313
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error